The Effect of Storage on the Accumulation of Oxidative Biomarkers in Donated Packed Red Blood Cells
The Effect of Storage on the Accumulation of Oxidative Biomarkers in Donated Packed Red Blood Cells
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DOI：
10.1097/ta.0b013e318191bfe0
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Bar-Or, David
中科院分区：
文献类型：
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作者：
Rael, Leonard T.;Bar-Or, Raphael;Bar-Or, David
背景：输血相关急性肺损伤（TRALI）是一种以氧化应激为特征的危及生命的疾病。较长的储存时间的压缩红细胞（PRBC）和其他血液制品已牵连与风险增加，在发展TRALI transfused patients.Methods：一个总共10个单位的血液中含有PRBC存储在柠檬酸盐-磷酸盐-葡萄糖缓冲液在VC中被列入研究。在Bonfils血液中心（Denver，CO），在储存第1天和第42天采集样本。立即离心样品，收集上清液并储存在-80 °C下直至进一步分析。结果：与第1天的样本（62.6 mV +/-21.5 SD）相比，第42天的样本（98.1 mV +/-21.9 SD）的氧化还原电位显著增加（p <0.05）。人血清白蛋白的氧化在储存期间增加了63.6%。其他血清蛋白，如载脂蛋白A1和甲状腺素运载蛋白表现出类似的氧化增加。此外，蛋白质与切割的C-末端氨基酸进行观察，表明存在的羧肽酶活性，一个标记inflammation.Conclusions：在输血PRBC的氧化环境的存在下，随着储存时间的增加。这可能部分解释了与输注较旧血液制品相关的TRALI风险增加。
Background: Transfusion-related acute lung injury (TRALI) is a life-threatening condition characterized by oxidative stress. Longer storage times of packed red blood cells (PRBC) and other blood products have been implicated with an increased risk in developing TRALI in transfused patients.Methods: A total of 10 units of blood containing PRBC stored in citrate-phosphate-dextrose buffer at VC were included in the study. At Bonfils Blood Center (Denver, CO), samples were collected on storage day 1 and day 42. Samples were immediately centrifuged, and the supernatants were collected and stored at -80 degrees C until further analysis. Oxidation-reduction potential and protein oxidation were measured in both the day 1 and day 42 samples.Results: Oxidation-reduction potential significantly increased (p < 0.05) in the day 42 sample (98.1 mV +/- 21.9 SD) versus the day 1 sample (62.6 mV +/- 21.5 SD). The oxidation of human serum albumin increased by 63.6% during the storage time. Other serum proteins such as apolipoprotein A1 and transthyretin demonstrated similar increases in oxidation. Also, proteins with a cleaved C-terminal amino acid were observed indicating the presence of carboxypeptidase activity, a marker of inflammation.Conclusions: The presence of an oxidative environment in transfused PRBC increases with storage time. This could partially explain the increased risk of developing TRALI related to the transfusion of older blood products.