A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells
A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells
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DOI：
10.1139/bcb-2013-0002
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发表时间：
2013-08-01
影响因子：
2.9
通讯作者：
Lin, Xiu-kun
中科院分区：
文献类型：
--
作者：
Wang, Feng-xia;Wu, Ning;Lin, Xiu-kun
中华地鳖步行者是一种重要的中药昆虫。在这项研究中，我们纯化了一个72 kDa的抗癌蛋白，指定为EPS 72，从这个物种使用硫酸铵沉淀，超滤，CM琼脂糖凝胶快速流阳离子交换，Q琼脂糖凝胶高性能（HP）阴离子交换，丁基琼脂糖凝胶HP疏水色谱，Superdex 75凝胶过滤色谱技术。EPS 72对人肺癌A549细胞系表现出有效的抗癌活性（IC 50，18.76 μ g/mL）。进一步研究表明，EPS 72可诱导A549细胞脱落和凋亡，抑制细胞与纤连蛋白和IV型胶原的粘附，抑制细胞的迁移和侵袭。此外，EPS 72显著降低β 1-整合素的表达。这项研究表明，由于其可能的抗转移活性，EPS 72可能被开发为一种新的抗癌治疗剂。
Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.