TRANSFER OF HEPARIN ACROSS THE HUMAN PERFUSED PLACENTAL LOBULE
TRANSFER OF HEPARIN ACROSS THE HUMAN PERFUSED PLACENTAL LOBULE
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DOI：
10.1111/j.2042-7158.1992.tb07073.x
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发表时间：
1992-12-01
影响因子：
3.3
通讯作者：
CONTRACTOR, SF
中科院分区：
文献类型：
--
作者：
BAJORIA, R;CONTRACTOR, SF
使用足月人胎盘分离小叶的双重灌注系统作为模型来研究肝素从母体循环至胎儿循环的转移。通过测量母体和胎儿灌注液中的 β-HCG 和碱性磷酸酶水平来评估系统的代谢活力。使用肌酐和安替比林作为标记物来确定母体和胎儿循环的并置。这项研究的结果表明，在母体循环中单次推注剂量的肝素后，其浓度从 15 分钟时的 99.01 +/- 2.98 缓慢下降至 97.23 +/- 4-12%，胎儿循环中肝素的转移呈线性，并在 15 分钟内从 15 分钟时的 0.10% +/- 0.05% 增加到 0-46 +/- 0.19%。 120 分钟发现母体 (MAUC) 和胎儿 (FAUC) 浓度-时间积分分别为 70160 +/- 1332 和 340 +/- 30 int。单位分别为 min mL-1。肝素和肌酐的胎盘渗透性（以胎儿浓度与母胎整体浓度差之比计算）分别为 8.65 x 10(-5) +/- 0.80 x 10 (-5) 和 0.033 +/- 0.006 mL min-1 g-1 灌注胎盘重量。这些数据表明肝素少量地从母体转移到胎儿循环中。
A system of dual perfusion of an isolated lobule of term human placenta was used as a model to study the transfer of heparin from maternal to foetal circulation. The metabolic viability of the system was assessed by measuring beta-HCG and alkaline phosphatase levels in both maternal and foetal perfusates. Creatinine and antipyrine were used as markers to determine juxtaposition of the maternal and foetal circulations. Results of this study indicate that following administration of a single bolus dose of heparin into the maternal circulation, its concentration declined slowly from 99.01 +/- 2.98 at 15 min to 97.23 +/- 4-12% and transfer of heparin in the foetal circulation was linear and increased from 0.10% +/- 0.05% at 15 min to 0-46 +/- 0.19% over a period of 120 min. The maternal (MAUC) and foetal (FAUC) concentration-time integrals were found to be 70160 +/- 1332 and 340 +/- 30 int. units min mL-1, respectively. Placental permeability of heparin and creatinine, calculated as the ratio of foetal concentration to the integral maternal-foetal concentration difference, was 8.65 x 10(-5) +/- 0.80 x 10 (-5) and 0.033 +/- 0.006 mL min-1 g-1 of perfused placental weight, respectively. These data suggest that heparin was transferred from the maternal to the foetal circulation in small quantities.