Renewable and nonrenewable resources: Amino acid turnover and allocation to reproduction in lepidoptera
Renewable and nonrenewable resources: Amino acid turnover and allocation to reproduction in lepidoptera
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DOI：
10.1073/pnas.072346699
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发表时间：
2002-04-02
影响因子：
11.1
通讯作者：
Boggs, CL
中科院分区：
文献类型：
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作者：
O'Brien, DM;Fogel, ML;Boggs, CL
动物生殖过程中营养资源的分配是一个具有重要进化意义的复杂过程。我们使用特定化合物的碳稳定同位素分析（GC/燃烧/同位素比MS）来研究食蜜天蛾卵氨基酸的膳食来源。以前的研究表明，鸡蛋制造中使用的营养素分为两类：在雌性的一生中越来越多地从成年饮食糖中合成的营养素（可再生资源），以及那些仅保留幼虫来源的营养素（不可再生资源）。我们预测，非必需氨基酸和必需氨基酸对应于这些营养素类和测试这种预测分析鸡蛋的氨基酸从女性喂养的同位素不同的饮食作为幼虫和成人。结果表明，必需的鸡蛋氨基酸完全来自幼虫的饮食。相反，非必需的鸡蛋氨基酸越来越多地合成成人膳食糖，在整个女性的一生营业额模式。这项研究表明，雌性鳞翅目昆虫可以利用内源氮源从花蜜糖合成大部分卵氨基酸。然而，必需氨基酸仅来源于幼虫的饮食，这限制了成年食物资源的使用，以提高繁殖成功率。
The allocation of nutritional resources to reproduction in animals is a complex process of great evolutionary significance. We use compound-specific stable isotope analysis of carbon (GC/combustion/isotope ratio MS) to investigate the dietary sources of egg amino acids in a nectar-feeding hawkmoth. Previous work suggests that the nutrients used in egg manufacture fall into two classes: those that are increasingly synthesized from adult dietary sugar over a female's lifetime (renewable resources), and those that remain exclusively larval in origin (nonrenewable resources). We predict that nonessential and essential amino acids correspond to these nutrient classes and test this prediction by analyzing egg amino acids from females fed isotopically distinct diets as larvae and as adults. The results demonstrate that essential egg amino acids originate entirely from the larval diet. In contrast, nonessential egg amino acids were increasingly synthesized from adult dietary sugars, following a turnover pattern across a female's lifetime. This study demonstrates that female Lepidoptera can synthesize a large fraction of egg amino acids from nectar sugars, using endogenous sources of nitrogen. However, essential amino acids derive only from the larval diet, placing an upper limit on the use of adult dietary resources to enhance reproductive success.