ULTRASOUND ENHANCED ENDOCYTOTIC ACTIVITY OF HUMAN FIBROBLASTS
ULTRASOUND ENHANCED ENDOCYTOTIC ACTIVITY OF HUMAN FIBROBLASTS
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DOI：
10.1016/j.ultrasmedbio.2009.06.1090
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发表时间：
2009-12-01
影响因子：
2.9
通讯作者：
Hauser, Manfred
中科院分区：
文献类型：
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作者：
Hauser, Joerg;Ellisman, Mark;Hauser, Manfred
尽管各种体外研究表明，低强度脉冲超声影响细胞骨架成分和生化途径，但确切的生物学机制仍不完全清楚。在这项研究中，我们分析了治疗性超声对人包皮成纤维细胞内吞活性的影响。将成纤维细胞与两种不同的内吞标记物（转铁蛋白Alexa 488和荧光黄; Sigma Bioprobes，尤金，OR，美国）一起孵育。为了评价超声处理和非超声处理对照细胞中内化标志物的量，进行共聚焦显微镜和读板器实验。此外，通过电子显微镜监测细胞膜的结构完整性。超声处理后，检测到荧光标记物摄取的明显增加（1.6倍/荧光黄和1.4倍/转铁蛋白Alexa 488）。共聚焦显微镜和酶标仪实验显示，与未超声处理的细胞相比，超声处理的成纤维细胞的整个群体显示出显著更高的荧光（p
Although various in vitro studies have shown that low-intensity pulsed ultrasound influences cytoskeletal components and biochemical pathways, the exact biologic mechanisms are still not fully understood. In this study, we analysed the effect of therapeutic ultrasound on the endocytotic activity of human foreskin fibroblasts. Fibroblasts were incubated with two different endocytotic markers (transferrin Alexa 488 and Lucifer yellow; Sigma Bioprobes, Eugene, OR, USA). To evaluate the amount of internalized markers in sonicated and nonsonicated control cells, confocal microscopy and plate reader experiments were performed. Additionally, the structural integrity of the cell membrane was monitored by electron-microscopy. After ultrasound treatment a clear increase (1.6-fold/Lucifer yellow and 1.4-fold/transferrin Alexa 488) of fluorescent marker uptake was detected. Confocal microscopy and plate reader experiments revealed that whole populations of sonicated fibroblasts showed a significant higher fluorescence compared with cells not sonicated (p