Conversion of angiotensin-1 to angiotensin-2 by a latent endothelial cell peptidyl dipeptidase that is not angiotensin-converting enzyme.
Conversion of angiotensin-1 to angiotensin-2 by a latent endothelial cell peptidyl dipeptidase that is not angiotensin-converting enzyme.
复制标题
潜在的内皮细胞肽基二肽酶（不是血管紧张素转换酶）将血管紧张素 1 转化为血管紧张素 2。
DOI：
10.1016/s0006-291x(86)80487-9
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Fanburg,BL
中科院分区：
文献类型：
--
作者：
Lanzillo,JJ;Dasarathy,Y;Stevens,J;Fanburg,BL
培养的牛肺动脉内皮细胞含有与血管紧张素转换酶不同的第二肽基二肽酶，它以非活性形式存在，与不可透析的抑制物有关。用甘氨酸亲和层析部分纯化，将酶和抑制剂分离，得到一种能水解血管紧张素-1、缓激肽、P物质、心房肽-2、脑啡肽和Hip-His-Leu的制剂。该酶不受赖诺普利、卡托普利、硫胺、磷酰胺、大豆胰酶抑制剂、PMSF、氨基肽酶和羧肽酶抑制剂的抑制，但受EDTA抑制。
Cultured bovine pulmonary artery endothelial cells contain a second peptidyl dipeptidase, distinct from angiotensin-converting enzyme, present in an inactive form associated with a non-dialyzable inhibitor. Partial purification by glycine affinity chromatography separates enzyme from inhibitor to yield a preparation which hydrolyzes angiotensin-1, bradykinin, substance P, atriopeptin-2, enkephalin and Hip-His-Leu. This enzyme is resistant to inhibition by lisinopril, captopril, thiorphan, phosphoramidon, soybean trypsin inhibitor, PMSF and aminopeptidase and carboxypeptidase inhibitors, but is inhibited by EDTA.