Characterizing distribution of steroidal alkaloids in Fritillaria spp. and related compound formulas by liquid chromatography-mass spectrometry combined with hierarchial cluster analysis
Characterizing distribution of steroidal alkaloids in Fritillaria spp. and related compound formulas by liquid chromatography-mass spectrometry combined with hierarchial cluster analysis
复制标题
DOI：
10.1016/j.chroma.2008.03.093
复制
发表时间：
2009-03-13
影响因子：
4.1
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Li, Hui-Jun;Jiang, Yan;Li, Ping
贝母球茎（Bulbus Fritillariae， BF）是百合科贝母属植物的总称，是一种常用的止咳祛痰中药。由于中药市场上BF植物来源的复杂性，迫切需要开发一种可靠的物种鉴定方法。以往基于形态学、组织学和分子生物学技术的研究都有各自的局限性。采用高效液相色谱-质谱（HPLC-MS）方法，对17种贝母中27种甾体生物碱和12种含bff的复方进行了鉴定和表征。利用估计的甾体生物碱相对组成，采用层次聚类分析对贝母物种进行化学分类研究。此外，还比较了所检查碱基的特征发生模式，以区分含bf的化合物配方的植物来源。结果表明，甾体生物碱的定性和定量差异不仅可用于某些药用贝母物种的化学分类，也可用于复方的物种鉴定。所描述的方法对含BF中药制剂的质量控制具有重要意义，可以防止BF混淆，也可以揭示掺假的可能存在。(C) 2008 Elsevier B.V.版权所有
Bulbus Fritillariae (BF), referred to the bulbs of several Fritillaria species (Liliaceae), is a commonly used antitussive and expectorant herb in traditional Chinese medicine (TCM). Due to the complexity of BF botanical origin in the herbal markets, it is urgently needed to develop a reliable method for species identification. Previous studies based on morphological and histological as well as molecular biological techniques have respective limitations. For the purpose of finding a possible discriminant method among Fritillaria species, 27 steroidal alkaloids in 17 Fritillaria species and 12 BF-containing compound formulas were identified and characterized by a high-performance liquid chromatography with mass spectrometry (HPLC-MS) method. The estimated relative composition of steroidal alkaloids was used to carry out a chemotaxonomical study on Fritillaria species by means of hierarchial cluster analysis. In addition, the characteristic occurring patterns of the examined bases were compared in an effort to distinguish the botanical origin of BF-containing compound formulas. The results demonstrated that the qualitative and quantitative differences in steroidal alkaloids were useful not only for chemotaxonomy in some medicinal Fritillaria species but also for species identification in compound formulas. The described method has important implications in quality control of BF-containing TCM preparations, allowing for the prevention of BF confusion, and also revealing the possible presence of adulteration. (C) 2008 Elsevier B.V. All rights reserved.