The olfactory bulb is rich in TRH immunoreactivity.
The olfactory bulb is rich in TRH immunoreactivity.
复制标题
嗅球富含 TRH 免疫反应性。
DOI：
10.1016/0006-8993(81)90185-2
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Krieger,NR
中科院分区：
文献类型：
--
作者：
Kreider,MS;Winokur,A;Krieger,NR
我们报道大鼠嗅球中有丰富的促甲状腺激素释放激素(TRH)免疫反应。TRH含量按Bassiri和Utiger2放射免疫法测定。嗅球TRH的平均浓度(60±10pg/mg湿重)是下丘脑的23%，至少是其他脑区的两倍。2个嗅球(平均湿重65 mg/2球)中TRH含量为3.9±0.3 ng。TRH免疫反应性可分为高分子形式和低分子形式。低分子形式与正品TRH(摩尔重量)共层析。362)，并能使体外孵育的垂体组织释放促甲状腺激素。由于嗅球的神经元组织和功能已被很好地描述，对TRH在该区域的定位和代谢的研究可能有助于阐明该三肽在中枢神经系统中的作用。
We report that the rat olfactory bulb is rich in thyrotropin releasing hormone (TRH) immunoreactivity. TRH content was determined according to the radioimmunoassay method of Bassiri and Utiger2. The concentration (mean ± S.E.M.,n= 10) of TRH in olfactory bulb (60 ± 10pg/mg wet weight) was 23% of the concentration in the hypothalamus, and was at least twice that of other brain regions examined. The 2 olfactory bulbs (mean wet weight 65 mg/2 bulbs) contained 3.9 ± 0.3 ng TRH. The TRH immunoreactivity could be separated into high and low molecular weight forms. The low molecular weight form co-chromatographed with authentic TRH (mol.wt. 362) on gel filtration and thin layer adsorption chromatography and caused the release of thyrotropin from pituitary tissue incubated in vitro. Since the neuronal organization and functions of the olfactory bulb are well described, studies of the localization and metabolism of TRH in this region may help to clarify the role of this tripeptide in the central nervous system.