8DSS-promoted remineralization of demineralized dentin in vitro
8DSS-promoted remineralization of demineralized dentin in vitro
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8DSS 促进脱矿牙本质的体外再矿化
DOI：
10.1039/c5tb00764j
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Li, Jiyao
中科院分区：
文献类型：
--
作者：
Liang, Kunneng;Xiao, Shimeng;Li, Jiyao
牙本质磷蛋白（Dentin phosphoprotein，DPP）在人牙齿的生物矿化过程中起着极其重要的作用。重复的核苷酸序列的琥珀酸-丝氨酸-丝氨酸（DSS）是DPP的基本单位，含有8个重复的DSS（8DSS）的肽已被证明具有诱导脱矿釉质脱矿的能力。在这项工作中，我们将8DSS肽涂覆在完全脱矿的牙本质上，以评估8DSS肽涂层对牙本质再矿化的影响。将人牙本质样品用37%磷酸脱矿2 min，然后包被8DSS肽（1 mg mL（-1）），并使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）检测8DSS肽与脱矿牙本质的结合强度。然后将涂覆的牙本质样品浸入人工唾液中3周。采用扫描电子显微镜（SEM）、能谱仪（EDS）、X射线衍射仪（XRD）和ATR-FTIR对牙本质进行表征。纳米压痕和原子力显微镜（AFM）的机械性能的陶瓷化表面进行了测定。结果表明，8DSS肽与脱矿牙本质具有良好的结合力，并能诱导牙本质表面和牙本质小管内纳米晶的沉积。经8DSS涂覆的样品的机械性能得到显著改善。相比之下，几乎没有任何新生成的矿物质沉积在没有8DSS肽涂层的样品上。因此，8DSS肽可能是一种有前途的生物材料，用于修复脱矿的人牙本质。
Dentin phosphoprotein (DPP) plays an extremely important role in the biomineralization of human tooth. The repetitive nucleotide sequence of aspartate-serine-serine (DSS) is the fundamental unit within DPP, and peptides containing 8 repeats of DSS (8DSS) have been shown to possess the ability to induce remineralization of the demineralized enamel. In this work, we coated the 8DSS peptide on the completely demineralized dentin to evaluate the effect of 8DSS peptide coating on dentin remineralization. Human dentin samples were demineralized with 37% phosphoric acid for 2 min, and then the 8DSS peptide (1 mg mL(-1)) was coated and the binding strength of the 8DSS peptide to demineralized dentin was examined using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Then the coated dentin samples were immersed in artificial saliva for 3 weeks. After that, the remineralized dentin samples were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) analysis and ATR-FTIR. The mechanical properties of the remineralized surfaces were determined by nano-indentation and atomic force microscopy (AFM). The results showed that the 8DSS peptide had good binding strength to demineralized dentin and could induce nanocrystals precipitation both on the surfaces and within the dentinal tubules. The mechanical properties of the 8DSS-coated samples were significantly improved. In contrast, there were hardly any newly generated minerals deposited on the samples without 8DSS peptide coating. In conclusion, the 8DSS peptide may be a promising biomaterial for restoring the demineralized human dentin.