Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China
Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China
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DOI：
10.3354/ame047141
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发表时间：
2007-05
影响因子：
1.4
通讯作者：
Yu Yang;Min-xi Wan;Wu-yang Shi;Hong Peng;G. Qiu;Jizhong Zhou;Xue-duan Liu
中科院分区：
文献类型：
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作者：
Yu Yang;Min-xi Wan;Wu-yang Shi;Hong Peng;G. Qiu;Jizhong Zhou;Xue-duan Liu
对采自广东省大宝山矿的三个酸性矿井水样品进行了研究。除理化分析外，还通过扩增核糖体DNA限制性分析（ARP-RFLP）在遗传水平上描述了这些样品的细菌多样性和群落结构。从377个克隆中获得了60种不同的ARM 1模式，并将其作为操作分类单位（OTU）进行研究，然后对其进行重新扩增和测序。序列数据和系统发育分析表明，嗜酸氧化亚铁硫杆菌占样品LC中细菌群体的88.0%。然而，样品JX和FS含有更多样化的细菌菌落，例如氧化亚铁细杆菌（JX：16.9%，FS：39.1%）、嗜酸菌（JX：38.7%，FS：25.8%）和A.氧化亚铁（JX：12.1%，FS：10.2%）。这些差异以辛普森指数（IAD）的倒数为特征，并与AMD中亚铁和有毒离子的浓度相关。
Three samples of acid mine drainage (AMD) collected from Dabaoshan Mine (Guangdong Province, China) were studied. In addition to physico-chemical analyses, bacterial diversities and community structures of these samples were described at genetic level by amplified ribosomal DNA restriction analysis (ARDRA). A total of 60 different ARDRA patterns were obtained from 377 clones and were studied as operational taxonomic units (OTUs), which were re-amplified and sequenced. The sequence data and phylogenetic analysis showed that Acidithiobacillus ferrooxidans represented 88.0 % of the bacterial population in Sample LC. However, Samples JX and FS contained more diverse colonies of bacteria, such as Leptospirillum ferrooxidans (JX: 16.9%, FS: 39.1%), Acidiphilum sp. (JX: 38.7%, FS: 25.8%) and A. ferrooxidans (JX: 12.1%, FS: 10.2%). These diversities were characterized by the reciprocal of Simpson's index (IAD) and correlated with the concentrations of ferrous iron and toxic ions in AMD.