Semi-heuristic phase compensation in digital holographic microscopy for stable and accurate quantitative phase imaging of moving objects
Semi-heuristic phase compensation in digital holographic microscopy for stable and accurate quantitative phase imaging of moving objects
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DOI：
10.1016/j.optlaseng.2023.107937
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发表时间：
2024
影响因子：
4.6
通讯作者：
S. Obando-Vásquez;A. Doblas;Carlos Trujillo
中科院分区：
文献类型：
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作者：
S. Obando-Vásquez;A. Doblas;Carlos Trujillo
数字全息显微术（DHM）是一种从数字记录的强度图案中恢复微观样品散射的复杂波场的先进干涉技术。在离轴DHM中，挑战在于数字地生成参考波前复制品以补偿干涉波之间的倾斜。目前的方法来估计参考波前的参数依赖于蛮力网格搜索或算法。然而，暴力强制搜索是耗时的，不切实际的视频速率定量相位成像和分析，应用全息视频中的方法是有限的，因为相位背景水平偶尔在帧之间的变化。提出了一种半启发式相位补偿（SHPC）算法来解决这些挑战，以从远心离轴数字全息显微镜记录的全息图中重建全视场（FOV）中失真最小的相位图像。该方法是测试与美国空军测试目标，涂抹红细胞和活的人类精子。SHPC方法提供了精确的相位图作为参考蛮力方法，但在处理时间减少了92倍。此外，该方法进行了测试，重建实验全息视频的动态试样，获得稳定的相位值和最小的差异，在帧之间的背景。所提出的方法提供了最先进的相位重建与高精度和稳定性的全息视频，允许成功的XYZ跟踪单运动的精子细胞。
Digital holographic microscopy (DHM) is a cutting-edge interferometric technique to recover the complex wavefield scattered by microscopic samples from digitally recorded intensity patterns. In off-axis DHM, the challenge is digitally generating the reference wavefront replica to compensate for the tilt between the interfering waves. Current methods to estimate the reference wavefront's parameters rely on brute-force grid searches or heuristics algorithms. Whereas brute-forced searches are time-consuming and impractical for video-rate quantitative phase imaging and analysis, applying heuristics methods in holographic videos is limited since the phase background level occasionally changes between frames. A semi-heuristic phase compensation (SHPC) algorithm is proposed to address these challenges to reconstruct phase images with minimum distortion in the full field-of-view (FOV) from holograms recorded by a telecentric off-axis digital holographic microscope. The method is tested with a USAF test target, smearing red blood cells and alive human sperm. The SHPC method provides accurate phase maps as the reference brute-force method but with a 92-fold reduction in processing time. Furthermore, this method was tested for reconstructing experimental holographic videos of dynamic specimens, obtaining stable phase values and minimal differences in the background between frames. This proposed method provides state-of-the-art phase reconstructions with high accuracy and stability in holographic videos, allowing the successful XYZ tracking of single-moving sperm cells.