Application of a Fusiogenic Peptide GALA for Intracellular Delivery
Application of a Fusiogenic Peptide GALA for Intracellular Delivery
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DOI：
10.1007/978-1-60761-919-2_37
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Futaki, Shiroh
中科院分区：
文献类型：
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作者：
Nakase, Ikuhiko;Kogure, Kentaro;Futaki, Shiroh
为了增强治疗药物和基因的胞质递送，pH敏感和膜融合肽已被用作促进其内体逃逸的添加剂。 GALA 是一种由 Glu-Ala-Leu-Ala 重复序列组成的肽，旨在模拟病毒融合蛋白序列的功能，介导病毒基因从酸性内体逃逸到胞质溶胶。最近，该肽不仅被应用于提高阳离子脂质体的质粒DNA转染效率，而且还被用作多功能包膜型纳米器件（MEND）的功能分子。使用这种肽的优点还体现在，在阳离子脂质复合物存在的情况下，通过 GALA 货物缀合物的有效内体逃逸来进行蛋白质的胞质递送。本章提供了使用 GALA 肽有效胞质递送货物分子的方案。
To enhance the cytosolic delivery of therapeutic drugs and genes, pH-sensitive and membrane fusiogenic peptides have been employed as additives for facilitating their endosomal escape. GALA is such a peptide composed of repeating sequences of Glu-Ala-Leu-Ala, which are designed to mimic the function of viral fusion protein sequences that mediate escape of virus genes from acidic endosomes to the cytosol. Recently, the peptide has been applied not only for improving the transfection efficiency of plasmid DNAs using cationic liposomes, but also as functional molecules of multifunctional envelope-type nano-device (MEND). The advantage of employing this peptide was also exemplified by the cytosolic deliver), of proteins via efficient endosomal escape of the GALA cargo conjugates in the presence of cationic lipid complexes. This chapter provides protocols for the efficient cytosolic delivery of cargo molecules using the GALA peptide.