Role of mitochondria in the maintenance of platelet function during in vitro storage
Role of mitochondria in the maintenance of platelet function during in vitro storage
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DOI：
10.1111/j.1365-3148.2010.01065.x
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发表时间：
2011-06-01
影响因子：
1.5
通讯作者：
Inoue, M.
中科院分区：
文献类型：
--
作者：
Hayashi, T.;Tanaka, S.;Inoue, M.
宗旨/目标：血小板在体外储存过程中发生结构和生物化学变化，这些变化统称为血小板储存病变（PSL）。线粒体是一种重要的细胞器，不仅参与能量产生，而且参与细胞功能和活力的调节。这意味着一些血小板功能可能受线粒体调节;因此，保存线粒体功能对于维持储存的血小板浓缩物（PC）中的血小板质量可能很重要。这项工作描述了各种化合物对线粒体功能的影响，重要的是在体外储存的PCs.Methods的血小板质量的维护：PC储存在22摄氏度与温和的搅拌中存在或不存在的2，4-二硝基苯酚，抗霉素A，乙酰-L-肉毒碱和抗坏血酸。这些产品对血小板质量的影响进行了评估，通过分析葡萄糖和乳酸盐浓度，pH值的存储介质，形状的血小板，线粒体膜电位和去极化，表面表达的CD 62 P和胶原蛋白诱导的血小板aggregation.Results：2，4-二硝基苯酚和抗霉素A增加PSL水平，而乙酰-L-肉毒碱降低水平的pH值和线粒体去极化的变化。储存介质中的抗坏血酸导致胶原诱导的血小板聚集水平提高。然而，没有一个检查试剂抑制CD 62 P在platelet.Conclusions表达：这些结果表明，线粒体功能的保存是根本的，但不完全足够的，在储存过程中维持血小板在体外质量。进一步的研究是必要的，以开发在体外储存的PC中保存线粒体和血小板功能的方法。
Aims/Objectives: Platelets undergo structural and biochemical alterations during in vitro storage and these are collectively called platelet storage lesions (PSL). The mitochondrion is an important cell organelle involved not only in energy production but also in the regulation of cellular functions and viability. This implies that some platelet functions may be regulated by mitochondria; hence, preservation of mitochondrial functions may be important for the maintenance of platelet quality in stored platelet concentrates (PCs). This work describes the effects of various compounds on mitochondrial functions important for the maintenance of platelet quality in in vitro stored PCs.Methods: PCs were stored at 22 degrees C with gentle agitation in the presence or absence of 2,4-dinitrophenol, antimycin A, acetyl-l-carnitine and ascorbic acid. The effects of these products on platelet quality were assessed by analysing glucose and lactate concentrations, pH of the storage medium, shape of the platelets, mitochondrial membrane potential and depolarisation, surface expression of CD62P and collagen-induced platelet aggregation.Results: 2,4-Dinitrophenol and antimycin A increased PSL levels, whereas acetyl-l-carnitine reduced the level of changes in pH and mitochondrial depolarisation. Ascorbic acid in the storage medium resulted in improved levels of collagen-induced platelet aggregation. However, none of the examined reagents suppressed CD62P expression in platelets.Conclusions: These results suggest that preservation of mitochondrial function is fundamental, but not fully sufficient, for the maintenance of platelet in vitro quality during storage. Further research is necessary to develop methods for preserving both mitochondrial and platelet functions in in vitro stored PCs.