Defining the function of SUMO system in pod development and abiotic stresses in Peanut
Defining the function of SUMO system in pod development and abiotic stresses in Peanut
复制标题
定义 SUMO 系统在花生荚果发育和非生物胁迫中的功能
DOI：
10.1186/s12870-019-2136-9
复制
发表时间：
2019-12-29
影响因子：
5.3
通讯作者：
Li, Guowei
中科院分区：
文献类型：
--
作者：
Liu, Yiyang;Zhu, Jiao;Li, Guowei
研究背景小泛素样修饰蛋白（SUMO）对蛋白质的翻译后修饰在植物的发育过程和对非生物胁迫的应答中起着重要的作用。然而，对花生（Arachis hypogaeaL.）中的SUMO化知之甚少，世界主要食用豆类作物之一。本研究对花生二倍体祖先基因组中的SUMO化系统进行了分析。duranensisandA.伊帕人我们的研究结果表明，花生也编码一种新的II类同种型的SCE 1，这是以前报道的唯一存在于谷物。RNA-seq数据显示，SUMO化级联反应的核心组分SUMO 1/2和SCE 1基因表现出豆荚特异性表达模式，暗示豆荚发育过程中的协调调控。此外，转录本和缀合物的配置文件显示，SUMO化有显着的作用，在豆荚的发展。此外，SUMO共轭物的动态变化，观察到在响应非生物stress.ConclusionsThe鉴定和组织的花生SUMO系统揭示SUMO化在逆境防御和豆荚发育过程中起着重要作用。本研究为花生荚果发育机理的揭示和提高花生农艺产量提供了新的途径。
BackgroundPosttranslational modification of proteins by small ubiquitin like modifier (SUMO) proteins play an important role during the developmental process and in response to abiotic stresses in plants. However, little is known about SUMOylation in peanut (Arachis hypogaeaL.), one of the world’s major food legume crops. In this study, we characterized the SUMOylation system from the diploid progenitor genomes of peanut,Arachis duranensis(AA) andArachis ipaensis(BB).ResultsGenome-wide analysis revealed the presence of 40 SUMO system genes inA. duranensisandA. ipaensis. Our results showed that peanut also encodes a novel class II isotype of the SCE1, which was previously reported to be uniquely present in cereals. RNA-seq data showed that the core components of the SUMOylation cascadeSUMO1/2andSCE1genes exhibited pod-specific expression patterns, implying coordinated regulation during pod development. Furthermore, both transcripts and conjugate profiles revealed that SUMOylation has significant roles during the pod development. Moreover, dynamic changes in the SUMO conjugates were observed in response to abiotic stresses.ConclusionsThe identification and organization of peanut SUMO system revealed SUMOylation has important roles during stress defense and pod development. The present study will serve as a resource for providing new strategies to enhance agronomic yield and reveal the mechanism of peanut pod development.