P2X receptors are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei of rat hypothalamus
P2X receptors are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei of rat hypothalamus
复制标题
DOI：
10.1007/s00418-008-0493-9
复制
发表时间：
2009-01-01
影响因子：
2.3
通讯作者：
Xiang, Zhenghua
中科院分区：
文献类型：
--
作者：
Guo, Wei;Sun, Jihu;Xiang, Zhenghua
本研究采用双标荧光免疫组织化学方法研究了大鼠下丘脑视上核和室旁核中P2X受体蛋白的分布以及P2X受体与加压素和催产素的共定位。结果显示，含加压素的神经元在视上核表达P2X(2)、P2X(4)、P2X(5)和P2X(6)受体，含催产素的神经元表达P2X(2)、P2X(4)和P2X(5)受体。在室旁核中，含有加压素的神经元表达P2X(4)、P2X(5)和P2X(6)受体，而含有催产素的神经元表达P2X(4)受体。这项研究提供了第一个证据，证明 P2X 受体亚基在视上核和室旁核中含有加压素和催产素的神经元上存在差异表达，因此为大鼠下丘脑中嘌呤能和加压素能系统以及嘌呤能和催产素能系统之间可能的功能相互作用提供了坚实的神经解剖学基础。
In the present study, the distribution of P2X receptor protein and colocalization of P2X receptors with vasopressin and oxytocin in the supraoptic and paraventricular nuclei of rat hypothalamus was studied using double-labeling fluorescence immunohistochemistry. The results showed that vasopressin-containing neurons expressed P2X(2), P2X(4), P2X(5) and P2X(6) receptor and oxytocin-containing neurons expressed P2X(2), P2X(4) and P2X(5) receptors in the supraoptic nucleus. In the paraventricular nucleus, vasopressin-containing neurons expressed P2X(4), P2X(5) and P2X(6) receptors, while oxytocin-containing neurons expressed P2X(4) receptors. This study provides the first evidence that P2X receptor subunits are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei, and hence, provides a substantial neuroanatomical basis for possible functional interactions between the purinergic and vasopressinergic systems, and the purinergic and oxytocinergic systems in the rat hypothalamus.