[Changes in number and distribution of retinal ganglion cells after optic nerve crush in zebrafish].
[Changes in number and distribution of retinal ganglion cells after optic nerve crush in zebrafish].
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发表时间：
2002-06
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通讯作者：
L. X. Zhou;Z. Wang
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作者：
L. X. Zhou;Z. Wang
研究了视神经损伤后斑马鱼（Brachydanio rerio）视网膜神经节细胞数量和分布的变化。视网膜神经节细胞层大约有40000到56000个细胞。正常鱼的神经节细胞密度分为6类，细胞密度最高的区域（中心区域）位于视盘的颞区。在视神经损伤后再生早期，丢失细胞的比例逐渐增加。中心区域细胞密度下降最快。在再生后期，视神经再生过程中神经节细胞损失约20%。结果表明，细胞损失可能发生凋亡而非坏死。一波细胞损失始于中心区域，并逐渐向外周进一步扩散。造成这些变化的原因可能是由于视神经功能暂时中断，从挤压中恢复和视神经快速再生的能力。
Changes in number and distribution of retinal ganglion cells were studied after optic nerve crush in zebrafish (Brachydanio rerio) with retinal wholemount. There were approximately 40,000 to 56,000 cells in the retinal ganglion cell layer. The density of ganglion cells was divided into six classes and the area of highest cell density (central area) was located at the temporal area to the optic disc in normal fish. At the early regeneration stages after optic nerve crush, the percentage of lost cells increased gradually. Cell density had fallen first in the central area. At the late regeneration stages, there was an approximately 20% loss of ganglion cells during optic nerve regeneration. The results suggest that the loss of cells may undergo apoptosis rather than necrosis. A wave of cell loss started in the central area and spread progressively further into periphery. The reason caused these changes may be due to temporal interruption of optic nerve function, recovery from crush and the ability to quickly regenerate in optic nerve of the fish.