High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya)
High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya)
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DOI：
10.1186/1471-2164-7-226
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发表时间：
2006-09-04
期刊：
影响因子：
4.4
通讯作者：
Valenzuela, Jesus G.
中科院分区：
文献类型：
--
作者：
Kato, Hirotomo;Anderson, Jennifer M.;Valenzuela, Jesus G.
背景：白蛉唾液蛋白是开发利什曼原虫感染控制疫苗的潜在靶点；在这项工作中，我们验证了一个假设，即来自地理上遥远的杜波斯奇白蛉种群的唾液蛋白由于宿主免疫反应施加的压力而高度分化。利用从马里捕获的野生杜波斯奇蝇和最近从肯尼亚定植的同种蝇制备唾液腺cDNA文库。结果：转录组和蛋白质组分析鉴定了最丰富的唾液腺分泌蛋白。通过系统发育树分析鉴定了这些唾液蛋白的同源基因。此外，对这两个不同人群的同源物进行比较分析，发现了高水平的蛋白质同一性，包括所有这些唾液蛋白预测的MHC II类t细胞表位。结论：这些数据驳斥了同一白蛉种群在地理上不同的唾液蛋白高度分化的假设。他们还建议在潜在的基于唾液的载体疫苗中使用相同的物种特异性成分的潜力。
Background: Salivary proteins from sandflies are potential targets for exploitation as vaccines to control Leishmania infection; in this work we tested the hypothesis that salivary proteins from geographically distant Phlebotomus duboscqi sandfly populations are highly divergent due to the pressure exerted by the host immune response. Salivary gland cDNA libraries were prepared from wild-caught P. duboscqi from Mali and recently colonised flies of the same species from Kenya.Results: Transcriptome and proteome analysis resulted in the identification of the most abundant salivary gland-secreted proteins. Orthologues of these salivary proteins were identified by phylogenetic tree analysis. Moreover, comparative analysis between the orthologues of these two different populations resulted in a high level of protein identity, including the predicted MHC class II T-cell epitopes from all these salivary proteins.Conclusion: These data refute the hypothesis that salivary proteins from geographically distinct populations of the same Phlebotomus sandfly species are highly divergent. They also suggest the potential for using the same species-specific components in a potential vector saliva-based vaccine.