[Photochemical and spectral properties of photosystem-2 subchloroplast fragments, highly purified from photosystem-1 mixtures].
[Photochemical and spectral properties of photosystem-2 subchloroplast fragments, highly purified from photosystem-1 mixtures].
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[从光系统 1 混合物中高度纯化的光系统 2 亚叶绿体片段的光化学和光谱特性]。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
Kutiurin Vm
中科院分区：
文献类型：
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作者：
Shutilova Ni;Klimov Vv;Shuvalov Va;Kutiurin Vm
本文提出了一种从光系统1混合物中分离光系统2片段的简单方法，即洗涤剂和差速离心相结合的方法。分离的片段的特征在于P700的含量不显著（每10500个叶绿素分子中有一个分子）和在荧光的低温发射光谱中在685和735 nm处的带值的高比率（F685/F735=5.9）。光化学活性和荧光光诱导变化能力的数据证明，光系统2的活性保留在反应中心操作的水平和水的光氧化与氧释放。
A rather simple method of isolation of photosystem 2 fragments, which are highly purified from Photosystem 1 admixture, has been developed on the basis of combined action of detergents and differential centrifugation. The isolated fragments are characterized by insignificant content of P700 (one molecule per 10500 molecules of chlorophyll) and by high ratio of band values at 685 and 735 nm in the low temperature emission spectrum of fluorescence (F685/F735=5.9). The data on photochemical activity and ability for photoinduced changes in fluorescence prove that the activity of Photosystem 2 is retained both at the level of reaction centre operation and at that of water photooxidation with oxygen evolution.