Influence of the membrane glycoprotein and cholesterol of vesicular stomatitis virus on the dynamics of viral and model membranes: fluorescence studies.
Influence of the membrane glycoprotein and cholesterol of vesicular stomatitis virus on the dynamics of viral and model membranes: fluorescence studies.
复制标题
水泡性口炎病毒膜糖蛋白和胆固醇对病毒和模型膜动力学的影响：荧光研究。
DOI：
10.1021/bi00284a013
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Wagner,RR
中科院分区：
文献类型：
--
作者：
Pal,R;Wiener,JR;Barenholz,Y;Wagner,RR
Ranajit Pal, Jon R. Wiener, Yechezkel Barenholz, and Robert R. Wagner*摘要：采用差分偏振相荧光法、稳态荧光各向异性和发射寿命测量方法，研究了水疱性口炎病毒积分膜糖蛋白与重组脂质囊中磷脂双分子层的相互作用。用0-辛基葡萄糖苷溶解法从病毒膜中分离糖蛋白，并用洗涤剂透析法重组成脂质囊泡。荧光探针1,6 -二苯基- 1,3,5 -己三烯和1 -[4-（三甲基氨）苯基]-6-苯基- 1,3,5 -己三烯显示，在双棕榈酰磷脂酰胆碱囊泡中，糖蛋白在凝胶期使双分子层紊乱，在液晶期使双分子层有序。当将病毒糖蛋白重构为1-棕榈酰-2-油酰-磷脂酰胆碱囊泡时，在整个研究温度范围内，只观察到蛋白对脂质双分子层的有序作用。两种探针的寿命都被发现在蛋白质的存在下略微缩短。当糖蛋白重组成病毒总脂质囊泡时，没有观察到糖蛋白对脂质期动力学的大影响。胆固醇在决定
Ranajit Pal, Jon R. Wiener, Yechezkel Barenholz, and Robert R. Wagner* abstract: The interaction of the integral membrane glycoprotein of vesicular stomatitis virus with the phospholipid bilayer in reconstituted lipid vesicles was determined by dif-ferential polarized phase fluorometry, steady-state fluorescence anisotropy, and emission lifetime measurements. The glyco-protein was isolated from the viral membraneby solubilization with octyl 0-glucopyranoside and was reconstituted into lipid vesicles by the detergent dialysis method. The fluorescent probes 1, 6-diphenyl-1, 3, 5-hexatriene and l-[4-(trimethyl-ammonio) phenyl]-6-phenyl-1, 3, 5-hexatriene revealed that, in dipalmitoylphosphatidylcholine vesicles, the glycoprotein disorders the bilayer in the gel phase and orders it to a small extent in the liquid-crystalline phase. When the viral glyco-protein was reconstituted into 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles, only the ordering effect of the protein on the lipid bilayer was observed throughout the temperature range studied. The lifetime of both probes was found to be slightly shortened in the presence of the protein. No large effect of glycoprotein on the dynamics of the lipid phase was observed when the protein was reconstituted into total viral lipid vesicles. The role that cholesterol plays in determining