OsWS1 involved in cuticular wax biosynthesis is regulated by osa-miR1848.
OsWS1 involved in cuticular wax biosynthesis is regulated by osa-miR1848.
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DOI：
10.1111/pce.12576
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Kuai-fei Xia;Xiaojin Ou;Chunzhi Gao;H. Tang;Yongxia Jia;R. Deng;Xinlan Xu;Mingyong Zhang
中科院分区：
文献类型：
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作者：
Kuai-fei Xia;Xiaojin Ou;Chunzhi Gao;H. Tang;Yongxia Jia;R. Deng;Xinlan Xu;Mingyong Zhang
角质层蜡形成一层疏水层，覆盖在地上植物器官上，作为生物和非生物胁迫的保护屏障。与已知的蜡生物合成途径相比，蜡生物合成的分子调控尚不清楚。在这里，我们发现水稻OsWS1是膜结合o -酰基转移酶基因家族的成员，参与蜡的生物合成，并受osa-miR1848的调控。osws1标记的绿色荧光蛋白定位于内质网（ER）。与野生型水稻相比，OsWS1过表达植株的总蜡质含量增加了3%，其中甚长链脂肪酸含量增加了35%，气孔周围的蜡质乳头更密集，叶片和茎表面形成更多的表皮蜡晶体，花粉外壳更厚，幼苗成活率更高。相比之下，OsWS1-RNAi和osa-miR1848过表达植物表现出相反的变化。基因表达分析显示，过表达osa-miR1848可下调OsWS1转录本；此外，OsWS1和osa-miR1848在叶片、穗部和茎部以及水分亏缺处理中的表达谱呈负相关。这些结果表明OsWS1受osa-miR1848调控，参与角质层蜡质的形成。
Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well-known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice OsWS1, a member of the membrane-bound O-acyl transferase gene family, involved in wax biosynthesis and was regulated by an osa-miR1848. OsWS1-tagged green fluorescent protein localized to the endoplasmic reticulum (ER). Compared with wild-type rice, OsWS1 overexpression plants displayed a 3% increase in total wax, especially a 35% increase in very long-chain fatty acids, denser wax papillae around the stoma, more cuticular wax crystals formed on leaf and stem surfaces, pollen coats were thicker and more seedlings survived after water-deficit treatment. In contrast, OsWS1-RNAi and osa-miR1848 overexpression plants exhibited opposing changes. Gene expression analysis showed that overexpression of osa-miR1848 down-regulated OsWS1 transcripts; furthermore, expression profiles of OsWS1 and osa-miR1848 were inversely correlated in the leaf, panicle and stem, and upon water-deficit treatment. These results suggest that OsWS1 is regulated by osa-miR1848 and participates in cuticular wax formation.