A theoretical method to determine unstressed off-rate from multiple bond force spectroscopy.
A theoretical method to determine unstressed off-rate from multiple bond force spectroscopy.
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一种从多重键力光谱确定无应力解离速率的理论方法。
DOI：
10.1016/j.colsurfb.2012.02.010
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Eggleton,CD
中科院分区：
文献类型：
--
作者：
Gupta,VK;Eggleton,CD
使用动态力光谱来测量分子间键的动力学解离速率目前需要分离单个分子。出现这种要求的部分原因是，目前还没有一种易于处理的分析方法来确定动力学解离率，从动态力下的大量债券的断裂。我们介绍了一种新的方法来确定无应力的解离速率从动态力谱实验涉及大量的债券。使用Bell和Dembo模型，我们表明，在初始键数（50-500）和加载速率（103- 106 pN/s）下，使用所提出的方法计算的无应力解离速率与多键动态力谱实验的蒙特-卡罗模拟中使用的规定的无应力解离速率是一致的。
Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 103–106pN/s.