Screening of chlorina mutants of barley (Hordeum vulgare L.) with antibodies against light-harvesting proteins of PS I and PS II: Absence of specific antenna proteins
Screening of chlorina mutants of barley (Hordeum vulgare L.) with antibodies against light-harvesting proteins of PS I and PS II: Absence of specific antenna proteins
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DOI：
10.1023/a:1005823711838
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发表时间：
1997-05-01
影响因子：
3.7
通讯作者：
Jansson, S
中科院分区：
文献类型：
--
作者：
Bossmann, B;Knoetzel, J;Jansson, S
利用单特异性抗体和单克隆抗体对23个来自哥本哈根嘉士伯实验室大麦突变体库的chlorina（clo）突变体进行了光系统I（Lhcal-4）和PS II天线蛋白Lhcb 1 -3（LHC II）、Lhcb 4 -6（CP 29、CP 26、CP 24）和PsbS（CP 22）的4种捕光叶绿素（Chl）a/b结合蛋白（LHC）的检测。与大麦突变体clo-f2等位的突变体在Chi B合成中受损，提供了证据表明Lhca 4、Lhcb 1和Lhcb 6在Chi B不存在的情况下是不稳定的，并且Lhcb 2、Lhcb 3和Lhcb 4的积累也受损。chlorina-a基因座的突变体（clo-a（117）、clo-a（126）和clo-a（134））缺乏或仅具有痕量的Lhca 1、Lhca 4、Lhcb 1和Lhcb 3，而chlorina-b基因座的突变体（clo-b（125））具有减少量的所有Lhca蛋白。这两个突变可能会影响蛋白质的输入或组装。有证据表明，Lhcb 5是PS II的最内层LHC蛋白，Lhca 1和Lhca 4，这被认为是密切相关的LHCI-730复合物，可以积累彼此独立。从clo-f2（101）、clo-a（126）和clo-b（125）的叶片获得的77 K荧光发射光谱表明，在742 nm处发射的叶绿素与结合到反应中心的Lhca 4的存在耦合，并且那些在730 nm附近发射的叶绿素位于Lhca 1上。
Twenty-three chlorina (clo) mutants from the barley mutant collection of the Carlsberg Laboratory, Copenhagen, were tested for the presence of the four light-harvesting chlorophyll (Chl) a/b-binding proteins (LHC) of Photosystem I (Lhcal-4) and the PS II antenna proteins Lhcb1-3 (LHC II), Lhcb4-6 (CP29, CP26, CP24) and PsbS (CP22) using monospecific and monoclonal antibodies. Mutants allelic to barley mutant clo-f2, impaired in Chi b synthesis, provided evidence that Lhca4, Lhcb1 and Lhcb6 are unstable in the absence of Chi b, and the accumulation of Lhcb2, Lhcb3 and Lhcb4 is also impaired. Mutants at the locus chlorina-a (clo-a(117), clo-a(126) and clo-a(134)) lack or have only trace amounts of Lhca1, Lhca4, Lhcb1 and Lhcb3, whereas a mutant at the locus chlorina-b (clo-b(125)) had reduced amounts of all Lhca proteins. These two mutations could have an effect in protein import or assembly. Evidence is presented that Lhcb5 is the innermost LHC protein of PS II, and that Lhca1 and Lhca4, which have been supposed to be intimately associated in the LHCI-730 complex, can accumulate independently of each other. 77 K fluorescence emission spectra taken from leaves of clo-f2(101), clo-a(126) and clo-b(125) indicate that chlorophyll(s) emitting at 742 nm are coupled to the presence of Lhca4 that is bound to the reaction centre, and those emitting around 730 nm are located on Lhca1.