Lipidic cubic phase technologies for membrane protein structural studies
Lipidic cubic phase technologies for membrane protein structural studies
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DOI：
10.1016/j.sbi.2011.06.007
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发表时间：
2011-08-01
影响因子：
6.8
通讯作者：
Cherezov, Vadim
中科院分区：
文献类型：
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作者：
Cherezov, Vadim
脂质立方相（LCP）是一种模拟膜的基质，适合于在微环境中稳定和结晶膜蛋白。然而，LCP技术还没有被膜蛋白结构生物学界完全接受，主要是因为与处理粘性材料相关的困难。最近的发展预结晶分析和晶体成像的改进，成功地获得高分辨率的G蛋白偶联受体（GPCR）的结构，和商业可用性的LCP工具和仪器开始吸引结构生物学家在他们的研究中整合LCP技术。这种更广泛的接受应转化为增加的数量，否则难以结晶膜蛋白结构，阐明其功能机制和脂质-蛋白质相互作用的结构细节。
Lipidic cubic phase (LCP) is a membrane-mimetic matrix suitable for stabilization and crystallization of membrane proteins in lipidic environment. LCP technologies, however, have not been fully embraced by the membrane protein structural biology community, primarily because of the difficulties associated with handling viscous materials. Recent developments of pre-crystallization assays and improvements in crystal imaging, successes in obtaining high resolution structures of G protein-coupled receptors (GPCRs), and commercial availability of LCP tools and instruments are beginning to attract structural biologists to integrate LCP technologies in their research. This wider acceptance should translate to an increased number of otherwise difficult-to-crystallize membrane protein structures, shedding light on their functional mechanisms and on structural details of lipid-protein interactions.