The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle.
The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle.
复制标题
DOI：
10.1073/pnas.98.3.980
复制
发表时间：
2001-01
影响因子：
11.1
通讯作者：
Xiaoli Fu;Y. Shih;Yan Zhang;L. Rothfield
中科院分区：
文献类型：
--
作者：
Xiaoli Fu;Y. Shih;Yan Zhang;L. Rothfield
将分裂位点置于大肠杆菌中的中间细胞需要MinE蛋白。MinE通过赋予MinCD分裂抑制剂拓扑特异性而起作用，防止抑制剂作用于中间细胞位点，同时允许其阻断沿细胞长度沿着的其他不需要的位点处的分裂。先前表明，MinE组装成似乎定位在中细胞附近的环状结构，显然解释了MinE在中细胞特异性抵消MinCD的能力。我们在这里报告说，MinE环不是固定在中间细胞附近的位置，而是一个动态的结构，经历了一个重复的运动周期，首先到一个细胞极，然后到相反的极。两者结合的动态行为的MinD蛋白的研究，结果表明，分裂位点的位置的拓扑特异性可能不涉及一个本地化的行动MinE抵消MinCD分裂抑制剂在中间细胞，而是MinE移动的能力分裂抑制剂远离中间细胞和细胞两极。
Placement of the division site at midcell in Escherichia coli requires the MinE protein. MinE acts by imparting topological specificity to the MinCD division inhibitor, preventing the inhibitor from acting at the midcell site while permitting it to block division at other unwanted sites along the length of the cell. It was previously shown that MinE assembled into a ring structure that appeared to be localized near midcell, apparently explaining the ability of MinE to specifically counteract MinCD at midcell. We report here that the MinE ring is not fixed in position near midcell but is a dynamic structure that undergoes a repetitive cycle of movement first to one cell pole and then to the opposite pole. Taken together with studies of the dynamic behavior of the MinD protein, the results suggest that the topological specificity of division site placement may not involve a localized action of MinE to counteract the MinCD division inhibitor at midcell but rather the ability of MinE to move the division inhibitor away from midcell and to the cell poles.