Mapping of c-Fos in the trigeminal sensory nucleus following high- and low-intensity afferent stimulation in the rat
Mapping of c-Fos in the trigeminal sensory nucleus following high- and low-intensity afferent stimulation in the rat
复制标题
大鼠高强度和低强度传入刺激后三叉神经感觉核中 c-Fos 的定位
DOI：
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发表时间：
2000
影响因子：
2
通讯作者：
Y. Shigenaga
中科院分区：
文献类型：
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作者：
M. Takemura;T. Shimada;S. Sugiyo;T. Nokubi;Y. Shigenaga
摘要 虽然以前的研究表明，三叉神经感觉核中口内和面结构的伤害性传入有不同的组织结构，但还需要更详细的数据。本研究旨在通过检测高强度和低强度电刺激加氏神经节(GG)后大鼠TSN内c-Fos表达的变化来填补这一空白。低强度刺激(0.1mA)可在口腔亚核背内侧亚核(Vo)、极间亚核背内侧亚核(Vi；120.5±40.1)、巨细胞带内侧角(VcIII/IV；47.5±10.5)和尾侧亚核(Vc)背内侧-腹外侧轴全长的浅层(VCi/II；1330.0±65.6)多个神经元中诱导c-Fos。在Vp主背亚核(10.0±4.9)和VcIII/IV外侧亚核(11.5±1.6)可见少量Fos阳性神经元。与低强度刺激相比，高强度刺激(1 MA)各亚区Fos阳性神经元数目显著增加(VP32.3±10.8；Vodm 270.3±75.3；Vidm 189.3±38.5；内侧VcIII/IV77.5±18.2；外侧VcIII/IV24.8±9.3；VCI/II，2155.8±470.2)。在低强度和高强度刺激下，Fos阳性神经元出现的区域均局限于吻侧亚核Vp、Vo和Vi的背侧或背内侧亚核，口内结构初级传入的主要投射野位于该亚核的背侧或背内侧亚核；Fos阳性神经元分布于整个VCI/II，沿Vc背内侧-腹外侧轴分布，面部皮肤初级传入的主投射野位于Vc背内侧-腹外侧轴。然而，诱导c-Fos的阈值因场而异。这些结果表明，口内区的伤害性加工是通过TSN的整个肩尾轴进行的，但主要是通过面部的VCI/II来进行。
Abstract Although previous studies have suggested that nociceptive afferents from intra-oral and facial structures are organized differently in the trigeminal sensory nucleus (TSN), more detailed data are needed. The present study aimed to fill this gap, by examining the changes in the expression of c-Fos within the rat TSN following high- and low-intensity electrical stimulation applied to the Gasserian ganglion (GG). A low-intensity stimulus (0.1 mA) induced c-Fos in many neurons in the dorsomedial subdivision (Vodm) of the oral subnucleus (Vo; mean±SEM in a certain segment=163.0±42.7), in the medial part of the dorsomedial subdivision (Vidm) of the interpolar subnucleus (Vi; 120.5±40.1), in the medial corner of the magnocellular zone (VcIII/IV; 47.5±10.5), and in the superficial layers (VcI/II; 1330.0±65.6) along the entire length of the dorsomedial-ventrolateral axis of the caudal subnucleus (Vc). A modest number of Fos-positive neurons were induced in the dorsal principal subnucleus (Vp; 10.0±4.9) and in the lateral VcIII/IV (11.5±1.6). A high-intensity stimulus (1.0 mA) significantly increased the number of Fos-positive neurons in each subdivision compared with the low-intensity stimulus (Vp 32.3±10.8; Vodm 270.3±75.3; Vidm 189.3±38.5; medial VcIII/IV 77.5±18.2; lateral VcIII/IV 24.8±9.3; VcI/II, 2155.8±470.2). At both low- and high-intensity stimulation, the fields where Fos-positive neurons appeared are restricted to the dorsal or dorsomedial subdivisions of the rostral subnuclei, Vp, Vo and Vi, where the main projectional fields of primary afferents from the intraoral structures are found, while Fos-positive neurons were distributed in the entire VcI/II, along the dorsomedial-ventrolateral axis of Vc, where the main projectional fields of primary afferents from the facial skin are found. The threshold to induce c-Fos is, however, different according to the fields. These results suggest that nociceptive processing in the intra-oral region is mediated through the entire length of the rostrocaudal axis of TSN, but is mediated primarily through VcI/II in the facial region.