Characterization of biofilm formed by human-derived nanoparticles.
Characterization of biofilm formed by human-derived nanoparticles.
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DOI：
10.2217/nnm.09.72
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Miller VM
中科院分区：
文献类型：
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作者：
Schwartz MK;Hunter LW;Huebner M;Lieske JC;Miller VM
微生物生物膜基质含有多糖和蛋白质，需要胞外核酸才能形成。实验的目的是识别人体动脉瘤中的感染性病原体，并表征钙化的人类动脉来源的纳米颗粒形成的生物膜。从48个炎症性动脉瘤中分离出26种不同的微生物病原体。将来自相似组织的相同数量(0.49麦克法兰单位)的纳米颗粒接种到24孔板中，在没有(对照)或存在核糖核酸酶、四环素或庆大霉素的情况下培养21天。对照生物膜在14天内形成，刀豆蛋白A和BacLight™绿染色检测到。核糖核酸酶处理的井中生物膜的形成与对照没有差异，但庆大霉素部分抑制生物膜的形成，四环素完全抑制生物膜的形成。因此，纳米颗粒生物膜保留了传统细菌生物膜的一些特征，并需要蛋白质-钙相互作用，尽管不需要细胞外RNA。该模型系统还可以研究来自供体组织的纳米大小的囊泡，包括任何存在的微生物，并可以为体外研究纳米颗粒生物膜的形成提供有用的工具。
Microbial biofilm matrix contains polysaccharides and proteins and can require extracellular nucleic acids for initial formation. Experiments were designed to identify infectious pathogens in human aneurysms and to characterize biofilm formed by calcified human arterial-derived nanoparticles. A total of 26 different microbial pathogens were isolated from 48 inflammatory aneurysms. Consistent amounts (0.49 McFarland units) of nanoparticles derived from similar tissue were seeded into 24-well plates and cultured for 21 days in the absence (control) or presence of RNase, tetracycline or gentamicin. Control biofilm developed within 14 days, as detected by concanavalin A and BacLight™ Green staining. The formation of biofilm in wells treated with RNase was not different from the control; however, gentamicin partially inhibited and tetracycline completely inhibited biofilm formation. Therefore, nanoparticle biofilm retains some characteristics of conventional bacterial biofilm and requires protein–calcium interactions, although extracellular RNA is not required. This model system may also allow study of nanosized vesicles derived from donor tissue, including any microbes present, and could provide a useful tool for in vitro investigation of nanoparticle biofilm formation.