Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.
Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.
复制标题
DOI：
10.1016/j.devcel.2015.10.001
复制
发表时间：
2015-10
影响因子：
11.8
通讯作者：
K. Shinohara;Duanduan Chen;T. Nishida;K. Misaki;S. Yonemura;H. Hamada
中科院分区：
文献类型：
--
作者：
K. Shinohara;Duanduan Chen;T. Nishida;K. Misaki;S. Yonemura;H. Hamada
小鼠胚胎左右不对称性的测定是通过顺时针旋转结节纤毛产生的微流体流来建立的。然而，结节纤毛如何实现稳定的单向旋转仍然是未知的。在这里，我们表明，短暂暴露于微管稳定药物紫杉醇（紫杉醇）诱导随机定向旋转和改变结纤毛的超微结构。在体内观察和计算机模拟表明，一个规则的9+0排列的双微管是必不可少的稳定的单向旋转的节点纤毛。气道9+2运动纤毛表现为平面搏动，对紫杉醇治疗有抵抗力。然而，缺乏放射状辐条头蛋白RSP4a的小鼠的气道纤毛经历旋转运动而不是平面跳动，易于微管重排，并且对紫杉醇敏感。我们的研究结果表明，没有径向辐条允许节点纤毛单向旋转，但作为一个权衡，使他们超微结构脆弱。
Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Taxol) induces randomly directed rotation and changes the ultrastructure of node cilia. In vivo observations and a computer simulation revealed that a regular 9+0 arrangement of doublet microtubules is essential for stable unidirectional rotation of node cilia. The 9+2 motile cilia of the airway, which manifest planar beating, are resistant to Taxol treatment. However, the airway cilia of mice lacking the radial spoke head protein Rsph4a undergo rotational movement instead of planar beating, are prone to microtubule rearrangement, and are sensitive to Taxol. Our results suggest that the absence of radial spokes allows node cilia to rotate unidirectionally but, as a trade-off, renders them ultrastructurally fragile.