DNA Microviscosity Characterization with Particle Diffusometry for Downstream DNA Detection Applications
DNA Microviscosity Characterization with Particle Diffusometry for Downstream DNA Detection Applications
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DOI：
10.1021/acs.analchem.7b03513
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发表时间：
2017-12-19
影响因子：
7.4
通讯作者：
Wereley, Steven T.
中科院分区：
文献类型：
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作者：
Clayton, Katherine N.;Berglund, Gregory D.;Wereley, Steven T.
DNA微粘度的分析表征为核拥挤、基于核酸的药物开发和基于核酸的生物传感器设备设计提供了重要的生物物理见解。然而，大多数粘度表征方法需要大量样品和破坏性测试。相比之下，粒子扩散法允许在原位分析DNA微粘度，测量时间短（8秒），使用小体积（
Analytical characterization of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid based pharmaceutical development, and nucleic acid based biosensor device design. However, most viscosity characterization methods require large sample volumes and destructive testing. In contrast, particle diffusometry permits in situ analysis of DNA microviscosity with short measurement times (8 s) using small volumes (