Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats
Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats
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电针ST36、GB39穴调节p53信号通路对佐剂性关节炎大鼠细胞凋亡的影响
DOI：
10.3892/mmr.2019.10674
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发表时间：
2019-11-01
影响因子：
3.4
通讯作者：
Zhu, Jun
中科院分区：
文献类型：
--
作者：
Su, Chengguo;Chen, Yuzhou;Zhu, Jun
p53和小鼠双微体2同源物（MDM 2）在滑膜细胞凋亡中起关键调节作用。本研究旨在观察电针“足三里"、”悬钟"穴对佐剂性关节炎（AA）大鼠细胞凋亡的影响。雄性SD大鼠40只，随机分为对照组、AA组、AA + EA组和AA +假EA组，每组10只。除对照组外，其余各组大鼠双侧后肢足垫注射完全弗氏佐剂，建立AA模型。AA + EA组电针ST 36、GB 39穴。AA +假电针组大鼠在距ST 36、GB 39穴位5 mm处进行经皮电刺激。记录各组大鼠关节炎指数评分和后肢爪体积。HE染色观察滑膜组织病理变化，TUNEL染色检测滑膜细胞凋亡率。此外，凋亡相关蛋白Bax、佛波醇-12-肉豆蔻酸酯-13-乙酸酯诱导的蛋白1（Noxa）和p53上调的凋亡调节因子（p53）的表达水平通过蛋白质印迹分析来确定。逆转录-定量聚合酶链反应（RT-qPCR）检测滑膜组织中p53和MDM 2基因及蛋白的表达; Western blot检测滑膜组织中p53和MDM 2蛋白的表达。结果表明，电针组大鼠关节炎指数评分和后肢爪体积较AA组明显减小（P
p53 and mouse double minute 2 homolog (MDM2) serve key regulatory roles in the apoptosis of synovial cells. The present study aimed to investigate the effects of electroacupuncture (EA) at the 'Zusanli' (ST36) and 'Xuanzhong' (GB39) acupoints on apoptosis in an adjuvant arthritis (AA) rat model. A total of 40 male Sprague-Dawley rats were randomly divided into Control, AA, AA + EA and AA + sham EA groups (n=10 rats in each group). Rats in all the groups, with the exception of the control group, were injected with Complete (TM) Freund's adjuvant into the bilateral hindlimb footpad to establish the AA model. Rats in the AA + EA group were treated with EA at the ST36 and GB39 acupoints. Rats in the AA + sham EA group were treated with percutaneous electrical stimulation at a position of 5 mm away from the ST36 and GB39 acupoints. The arthritis index scores and hindlimb paw volumes of the rats in each group were recorded. Subsequently, pathological changes in the synovial tissue were evaluated by hematoxylin and eosin (H&E) staining, and the apoptotic rate of the synovial cells was detected by TUNEL staining. In addition, the expression levels of the apoptosis-associated proteins, Bax, phorbol-12-myristate-13-acetate-induced protein 1 (Noxa) and p53 upregulated modulator of apoptosis (PUMA), were determined by western blot analysis. The expression of both the gene and protein of p53 and MDM2 in synovial tissue was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. The results indicated that the arthritis index scores and hindlimb paw volumes upon EA stimulation were significantly decreased compared with those of the AA group (P