Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron
Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron
复制标题
硫酸化杂/半乳岩藻聚糖多糖对多巴胺能神经元的结构-活性关系。
DOI：
10.1016/j.ijbiomac.2015.10.042
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发表时间：
2016-01-01
影响因子：
8.2
通讯作者：
Zhang, Quanbin
中科院分区：
文献类型：
--
作者：
Wang, Jing;Liu, Huaide;Zhang, Quanbin
帕金森病 (PD) 与多巴胺能神经元的进行性丧失和引起复杂症状的更广泛的神经元变化有关。本研究的目的是在体内和体外研究硫酸化杂多糖（DF1）和硫酸化半乳岩藻聚糖多糖（DF2）对多巴胺能神经元的构效关系。样品处理显着改善了 MPTP 诱导的 PD 小鼠中 DA 和 TH、Bcl-2 和 Bax 阳性神经元的消耗，DF1 显示出最高的活性。体外结果发现，DF1和DF2可以逆转MPP+引起的线粒体活性下降和LDL释放增加（P < 0.01或P < 0.001），这进一步证明DF1和DF2也对MPP+引起的神经元损伤发挥直接保护作用。此外，给予样品可有效降低 MPTP 小鼠的脂质过氧化并增加 GSH、GSH-PX、MDA 和 CAT 的水平/活性。因此，神经元保护作用可能部分是通过抗氧化活性和预防细胞凋亡来介导的。 DF1、DF2和DF的化学成分差异显着，其中DF1成分的化学成分最复杂，神经元保护活性最高。这些结果表明，不同的单糖和糖醛酸可能有助于神经元保护活性。 (C) 2015 Elsevier B.V. 保留所有权利。
Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms. The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro. Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity. The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP+ (P < 0.01 or P < 0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP+. Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice. Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis. The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity. These results suggest that diverse monosaccharides and uronic acid might contribute to neuron protective activity. (C) 2015 Elsevier B.V. All rights reserved.