Phenotypic characterization of the infiltrating immune cells in normal prostate, benign nodular prostatic hyperplasia and prostatic adenocarcinoma
Phenotypic characterization of the infiltrating immune cells in normal prostate, benign nodular prostatic hyperplasia and prostatic adenocarcinoma
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DOI：
10.1016/j.yexmp.2008.11.010
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发表时间：
2009-04-01
影响因子：
3.6
通讯作者：
Musalam, Adel O.
中科院分区：
文献类型：
--
作者：
Hussein, Mahmoud-Rezk A.;Al-Assiri, Mana;Musalam, Adel O.
背景：免疫细胞浸润在正常前列腺、良性结节性前列腺增生症和前列腺癌中是一种恒定的特征。本研究对正常前列腺、良性结节性前列腺增生症和前列腺癌的免疫细胞进行了详细的研究。假设：良性结节性前列腺增生症和前列腺癌的发生与免疫细胞的数量变化有关。材料和方法：采用免疫组织化学方法和小鼠单抗修饰T细胞(CD3)、组织细胞(CD68)和B淋巴细胞(CD20)对50例正常前列腺、良性结节前列腺增生症和高级别前列腺癌的免疫细胞密度和表型进行了检测。结果：免疫细胞渗出物由T细胞、CD68细胞和B淋巴细胞组成。组织细胞和B淋巴细胞。CD(+)3T淋巴细胞和CD68(+)细胞是主要的细胞群。我们观察了正常前列腺、良性结节性前列腺增生症和高级别前列腺癌中免疫细胞密度的变化。良性结节性前列腺增生症免疫细胞密度明显高于正常前列腺组织(3.4+/-0.4vs13.5+/-1.0，P
Background: Immune cell infiltrate is a constant feature in normal prostate, benign nodular prostatic hyperplasia and prostatic adenocarcinoma. This study elaborates on the cells of the immune system present in normal prostate, benign nodular prostatic hyperplasia and prostatic adenocarcinoma.Hypothesis: Here, we hypothesized that "the development of benign nodular prostatic hyperplasia and prostatic adenocarcinoma is associated with numeric alterations of the immune cell infiltrate".Materials and methods: A total of 50 transurethral prostatic resection specimens, each entailing normal prostate, benign nodular prostatic hyperplasia and high grade prostatic adenocarcinoma were evaluated for the density and phenotype of the immune cells using immunohistological methods and mouse monoclonal antibodies decorating T cells (CD3), histiocytes (CD68) and B lymphocytes (CD20).Results: Immune cell infiltrate was composed of T cells, histiocytes and B-lymphocytes. CD(+)3 T lymphocytes and CD68(+) cells were the predominant cell populations. We observed variations in the density of the immune cells among the normal prostate, benign nodular prostatic hyperplasia and high grade prostatic adenocarcinoma. Compared with normal prostate, benign nodular prostatic hyperplasia had a statistically significant high density of immune cells (3.4 +/- 0.4versus 13.5 +/- 1.0, P