Microbial community structure and the relationship with soil carbon and nitrogen in an original Korean pine forest of Changbai Mountain, China
Microbial community structure and the relationship with soil carbon and nitrogen in an original Korean pine forest of Changbai Mountain, China
复制标题
长白山原始红松林微生物群落结构及其与土壤碳氮的关系
DOI：
10.1186/s12866-019-1584-6
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发表时间：
2019-09-13
期刊：
影响因子：
4.2
通讯作者：
Feng, Fujuan
中科院分区：
文献类型：
--
作者：
Liu, Minghui;Sui, Xin;Feng, Fujuan
背景阔叶红松混交林是全球温带森林的重要组成部分。土壤微生物是该森林生态系统生物地球化学循环的主要驱动力，与土壤中的碳(C)和氮(N)组分存在复杂的相互作用。结果调查了长白山红松阔叶混交林(699~1177 m)土壤微生物群落垂直结构，分析了土壤微生物群落与土壤C、N组分的关系。结果表明，不同海拔高度土壤微生物和革兰氏阴性菌(G-)、革兰氏阳性菌(G+)、真菌(F)、丛枝菌根真菌(AMF)和放线菌( )的总磷脂脂肪酸含量差异显著(p<；P005)。真菌PLFAs与细菌PLFAs(F/B)的比值在H1和H2处较高。微生物群落组成与地理距离的关系不呈距离衰减规律。细菌在不同地点(海拔)的变异系数最大。土壤总有机碳(TOC)、总氮(TN)、土壤含水率(W)和针叶树树高断面积与阔叶树种断面积之比是影响土壤微生物PLFA各亚类变化的主要因素。结构方程模型表明，TOC对细菌生物量有显著的直接影响，对细菌和真菌生物量的间接影响是通过土壤易氧化有机碳(ROC)实现的。土壤N组分与细菌和真菌生物量无显著相关性。结论海拔高度对土壤微生物G-、G+、F、AMF和放线菌的总PLFA(TPLFA)和PLFA有显著影响。细菌对海拔的变化比其他微生物更敏感。环境异质性是影响微生物群落结构地理分布格局的主要因素。TOC、TN、W和RBA是土壤微生物量变化的主要驱动因子。C组分是影响真菌和细菌生物量的主要因素，ROC是微生物来源C库的主要来源之一。
BackgroundThe broad-leaved Korean pine mixed forest is an important and typical component of a global temperate forest. Soil microbes are the main driver of biogeochemical cycling in this forest ecosystem and have complex interactions with carbon (C) and nitrogen (N) components in the soil.ResultsWe investigated the vertical soil microbial community structure in a primary Korean pine-broadleaved mixed forest in Changbai Mountain (from 699 to 1177 m) and analyzed the relationship between the microbial community and both C and N components in the soil. The results showed that the total phospholipid fatty acid (PLFA) of soil microbes and Gram-negative bacteria (G-), Gram-positive bacteria (G+), fungi (F), arbuscular mycorrhizal fungi (AMF), and Actinomycetes varied significantly (p< 0.05) at different sites (elevations). The ratio of fungal PLFAs to bacterial PLFAs (F/B) was higher at site H1, and H2. The relationship between microbial community composition and geographic distance did not show a distance-decay pattern. The coefficients of variation for bacteria were maximum among different sites (elevations). Total soil organic carbon (TOC), total nitrogen (TN), soil water content (W), and the ratio of breast-height basal area of coniferous trees to that of broad-leaved tree species (RBA) were the main contributors to the variation observed in each subgroup of microbial PLFAs. The structure equation model showed that TOC had a significant direct effect on bacterial biomass and an indirect effect upon bacterial and fungal biomass via soil readily oxidized organic carbon (ROC). No significant relationship was observed between soil N fraction and the biomass of fungi and bacteria.ConclusionThe total PLFAs (tPLFA) and PLFAs of soil microbes, including G-, G+, F, AMF, and Actinomycetes, were significantly affected by elevation. Bacteria were more sensitive to changes in elevation than other microbes. Environmental heterogeneity was the main factor affecting the geographical distribution pattern of microbial community structure. TOC, TN, W and RBA were the main driving factors for the change in soil microbial biomass. C fraction was the main factor affecting the biomass of fungi and bacteria and ROC was one of the main sources of the microbial-derived C pool.