On the presence of peptide synthetase and polyketide synthase genes in the cyanobacterial genus Nodularia
On the presence of peptide synthetase and polyketide synthase genes in the cyanobacterial genus Nodularia
复制标题
DOI：
10.1111/j.1574-6968.2001.tb10566.x
复制
发表时间：
2001-03-15
影响因子：
2.1
通讯作者：
Neilan, BA
中科院分区：
文献类型：
--
作者：
Moffitt, MC;Neilan, BA
节瘤菌素是一种由水华蓝藻物种Nodularia spumigena产生的肝毒素。应用简并PCR技术检测了节瘤藻有毒菌株中的多肽合成酶和聚酮合成酶基因。使用特定的引物组，肽合成酶和聚酮合酶基因同源物中检测到的nodularin生产菌株表明肽合成酶和聚酮合酶酶复合物的生物合成的nodularin的可能的作用。还通过蛋白磷酸酶2A抑制测定分析了从世界各地分离的节球藻属菌株的节球藻素的产生。蛋白磷酸酶抑制试验以及节球藻中肽合成酶和聚酮合成酶基因的分子检测可能是评估环境中产节球藻素蓝细菌的有用技术。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Nodularin is a hepatotoxin produced by the bloom-forming cyanobacterial species Nodularia spumigena. Putative peptide synthetase and polyketide synthase genes were detected in toxic strains of Nodularia by degenerate PCR. Using specific primer sets, peptide synthetase and polykelide synthase gene homologues were detected in nodularin-producing strains indicating a possible role of peptide synthetase and polyketide synthase enzyme complexes in the biosynthesis of nodularin. Strains of Nodularia isolated from around the world were also analyzed for the production of nodularin by the protein phosphatase 2A inhibition assay. The protein phosphatase inhibition assay and the molecular detection of peptide synthetase and polykelide synthase genes in Nodularia may be useful techniques for the assessment of nodularin-producing cyanobacteria in the environment. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.