A rice quantitative trait locus for salt tolerance encodes a sodium transporter
A rice quantitative trait locus for salt tolerance encodes a sodium transporter
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DOI：
10.1038/ng1643
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发表时间：
2005-10-01
期刊：
影响因子：
30.8
通讯作者：
Lin, HX
中科院分区：
文献类型：
--
作者：
Ren, ZH;Gao, JP;Lin, HX
作物的许多重要农艺性状，包括抗逆性，都是由数量性状基因座（QTL）控制的复杂性状。这些QTL的分离对改善世界农业有很大的希望，但仍是一项具有挑战性的任务。我们之前定位了一个水稻QTL SKC 1，该QTL在盐胁迫下维持耐盐品种中的K+稳态（1），这与早期发现K+稳态在耐盐性中很重要（2，3）一致。为了解该QTL的分子基础，我们通过图位克隆的方法分离了SKC 1基因，发现该基因编码HKT型转运蛋白。SKC 1在木质部导管周围的薄壁组织细胞中优先表达。电压钳分析表明，SKC 1蛋白是一种Na+选择性转运蛋白。生理学分析表明，SKC 1参与了盐胁迫下K+/Na+的动态平衡调节，为提高作物耐盐性提供了潜在的工具。
Many important agronomic traits in crop plants, including stress tolerance, are complex traits controlled by quantitative trait loci (QTLs). Isolation of these QTLs holds great promise to improve world agriculture but is a challenging task. We previously mapped a rice QTL, SKC1, that maintained K+ homeostasis in the salt-tolerant variety under salt stress(1), consistent with the earlier finding that K+ homeostasis is important in salt tolerance(2,3). To understand the molecular basis of this QTL, we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters. SKC1 is preferentially expressed in the parenchyma cells surrounding the xylem vessels. Voltage-clamp analysis showed that SKC1 protein functions as a Na+-selective transporter. Physiological analysis suggested that SKC1 is involved in regulating K+/Na+ homeostasis under salt stress, providing a potential tool for improving salt tolerance in crops.