Reduced sensitivity to paraquat evolves under selection with low glyphosate doses in Lolium rigidum
Reduced sensitivity to paraquat evolves under selection with low glyphosate doses in Lolium rigidum
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DOI：
10.1007/s13593-011-0012-6
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发表时间：
2011-07-01
影响因子：
7.3
通讯作者：
Powles, Stephen B.
中科院分区：
文献类型：
--
作者：
Busi, Roberto;Powles, Stephen B.
这是首次报道低剂量草甘膦的选择会导致百草枯抗性的转变。杂草品种对除草剂的抗性是对世界农业的严重威胁。我们报告了当重复选择低剂量时，遗传可变的异花授粉草本植物硬黑麦草的快速抗性进化。结果表明，经过三代筛选、草甘膦剂量低于标示剂量的刚性草甘草种群不仅表现出对草甘膦的抗性进化，而且对结构上无关的除草剂百草枯的敏感性发生了渐进性和伴随的转变。因此，降低百草枯敏感性是低剂量草甘膦反复选择的结果。在三次选择草甘膦的后代中，估计导致50%死亡的百草枯剂量(LD50)是未选择的敏感亲本的4倍。研究低于标签的除草剂剂量选择的进化结果有助于防止杂草种群的遗传变化，并维持世界农业中广泛使用的除草剂的药效。
This is the first report of low-dose glyphosate selection causing a shift towards paraquat resistance. Herbicide resistance in weed species is a serious threat to world agriculture. We report rapid resistance evolution in the genetically variable cross-pollinated grass weed Lolium rigidum when recurrently selected with low doses. Results show that an herbicide-susceptible L. rigidum population selected over three generations with below-label doses of glyphosate exhibited not only glyphosate resistance evolution but also a progressive and concomitant shift in sensitivity to the structurally unrelated herbicide paraquat. Thus, reduced paraquat sensitivity was a consequence of recurrent selection with glyphosate at low doses. In the three-time glyphosate-selected progeny, the estimated paraquat dose to cause 50% mortality (LD50) was 4-fold greater than for the unselected susceptible parent. Studying the evolutionary outcomes of below-label herbicide dose selection can help prevent genetic changes in weed populations and sustain the efficacy of herbicides widely used in world agriculture.