HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PAMAQUINE, PRIMAQUINE AND CARBOXY PRIMAQUINE IN CALF PLASMA USING ELECTROCHEMICAL DETECTION
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PAMAQUINE, PRIMAQUINE AND CARBOXY PRIMAQUINE IN CALF PLASMA USING ELECTROCHEMICAL DETECTION
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DOI：
10.1016/0378-4347(92)80370-6
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发表时间：
1992-08-07
期刊：
影响因子：
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通讯作者：
NISHIKAWA, M
中科院分区：
文献类型：
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作者：
ENDOH, YS;YOSHIMURA, H;NISHIKAWA, M
建立了小牛血浆中帕马喹、伯氨喹和羧基伯氨喹的高效液相色谱电化学检测方法。蛋白质用乙腈沉淀后，在5 μ m C18改性聚合物凝胶柱上用等度移动的相分离药物。所有三种化合物在血浆中的检测限为0.01 μ g/ml。该方法在药代动力学研究中的适用性，证明了通过确定的三种物质在小牛单剂量的帕马喹或伯氨喹的血浆浓度。
A high-performance liquid chromatographic method with electrochemical detection is described for quantification of pamaquine, primaquine and carboxy primaquine in calf plasma. After the proteins had been precipitated with acetonitrile, the drugs were separated on a 5-mu-m C18-modified polymer gel column with an isocratic mobile phase. The detection limit was 0.01-mu-g/ml in plasma for all three compounds. The applicability of the method in pharmacokinetic studies was demonstrated by determining the plasma concentrations of the three substances in calves administered a single dose of pamaquine or primaquine.