Structural Layers of Ex Vivo Rat Hippocampus at 7T MRI
Structural Layers of Ex Vivo Rat Hippocampus at 7T MRI
复制标题
7T MRI 下离体大鼠海马的结构层
作者：
Jeanine Manuella Kamsu;J. Constans;F. Lamberton;P. Courthéoux;P. Denise;B. Philoxène;Maëlle Coquemont;S. Besnard
在记忆神经生理学和癫痫、阿尔茨海默病、缺血和抑郁症等多种疾病的病理生理学研究中，应用于海马体的磁共振成像 (MRI) 具有挑战性。海马体是一个轮廓清晰的大脑结构，具有多层组织。海马层的成像仅限于少数研究，并且需要高磁场和梯度强度。我们在 7T MRI 上执行了一项常规 MRI 序列，以便可视化并描绘离体大鼠大脑中的多层海马结构。我们优化了体积三维 T2 快速获取松弛增强 (RARE) 序列，并量化了海马体及其最薄层之一（齿状回颗粒层）的体积。此外，我们还测试了钆被动染色，目的是减少采集时间并增加图像对比度。使用适当的设置，在大鼠海马体内分化出六个离散层。在海马本身或阿蒙角 (AH) 中：分化了 CA1 的东方层、锥体层、放射层和空洞分子层。齿状回：分子层和颗粒层清晰可见。用钆对一个大脑进行被动染色，将采集时间缩短了四倍，并改善了各层之间的分化。使用标准接收器表面线圈在 7T MRI 上优化的常规序列将使我们能够研究各种神经病理学大鼠模型（焦虑、癫痫、神经退行性变）中海马的结构层（信号和体积）。
Magnetic resonance imaging (MRI) applied to the hippocampus is challenging in studies of the neurophysiology of memory and the physiopathology of numerous diseases such as epilepsy, Alzheimer’s disease, ischemia, and depression. The hippocampus is a well-delineated cerebral structure with a multi-layered organization. Imaging of hippocampus layers is limited to a few studies and requires high magnetic field and gradient strength. We performed one conventional MRI sequence on a 7T MRI in order to visualize and to delineate the multi-layered hippocampal structure ex vivo in rat brains. We optimized a volumic three-dimensional T2 Rapid Acquisition Relaxation Enhancement (RARE) sequence and quantified the volume of the hippocampus and one of its thinnest layers, the stratum granulare of the dentate gyrus. Additionally, we tested passive staining by gadolinium with the aim of decreasing the acquisition time and increasing image contrast. Using appropriated settings, six discrete layers were differentiated within the hippocampus in rats. In the hippocampus proper or Ammon’s Horn (AH): the stratum oriens, the stratum pyramidale of, the stratum radiatum, and the stratum lacunosum moleculare of the CA1 were differentiated. In the dentate gyrus: the stratum moleculare and the stratum granulare layer were seen distinctly. Passive staining of one brain with gadolinium decreased the acquisition time by four and improved the differentiation between the layers. A conventional sequence optimized on a 7T MRI with a standard receiver surface coil will allow us to study structural layers (signal and volume) of hippocampus in various rat models of neuropathology (anxiety, epilepsia, neurodegeneration).
海马形成：阿尔茨海默病的解剖学和病理模式。
影响因子：
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作者：
VanHoesen,GW;Hyman,BT
通讯作者：
Hyman,BT
使用结构 MRI 测量大鼠海马、前扣带皮层和压后颗粒皮层的体积。
DOI：
10.1016/s1385-299x(02)00181-2
发表时间：
2002
期刊：
Brain research. Brain research protocols
影响因子：
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作者：
Wolf,OT;Dyakin,V;Vadasz,C;deLeon,MJ;McEwen,BS;Bulloch,K
通讯作者：
Bulloch,K