Cardiac changes following arteriovenous fistula creation in a mouse model.
Cardiac changes following arteriovenous fistula creation in a mouse model.
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DOI：
10.1177/11297298211026083
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发表时间：
2023-01
影响因子：
1.9
通讯作者：
Lee, Timmy
中科院分区：
文献类型：
--
作者：
Ingle, Kevin;Pham, Linh;Lee, Viangkaeo;Guo, Lingling;Isayeva-Waldrop, Tatyana;Somarathna, Maheshika;Lee, Timmy
动静脉瘘（AVF）的产生可能对心脏结构和功能产生负面影响，并影响心血管死亡率。本研究的目的是在小鼠AVF模型中发展和表征AVF产生后的心脏变化。采用C57BL/6小鼠颈动脉和颈静脉构建avf。以假手术AVF小鼠为对照组。在AVF和假手术小鼠创建前（基线）和创建后7天和21天进行2d超声心动图检查。小天狼星红染色左心室用于胶原蛋白的生成。与假手术小鼠相比，心脏输出量（CO）、左室舒张末期（LVEDD）和收缩末期（LVESD）直径、舒张末期（LVEDV）和收缩末期（LVESV）体积在第7天和第21天显著增加。从基线到21天，AVF组的CO、LVEDD、LVESD、LVEDV和LVESV也有显著增加，但假手术小鼠没有。与假手术小鼠相比，第21天AVF的射血分数和分数缩短显著降低。在第21天，与假手术的AVF小鼠相比，AVF小鼠心脏组织血管周围和间质区域的小天狼星红明显更突出。在我们的小鼠模型中，AVF的创建导致心脏变化，如心输出量增加，左心室扩张和心脏纤维化，同时显示射血分数和分数缩短降低。
Arteriovenous fistula (AVF) creation may negatively affect cardiac structure and function and impact cardiovascular mortality. The objective of this study was to develop and characterize the cardiac changes following AVF creation in a murine AVF model. AVFs were constructed using the carotid artery and jugular vein in C57BL/6 mice. Sham-operated AVF mice served as the control group. 2D-echocardiography was performed prior to AVF creation (baseline) and at 7 and 21 days after creation in AVF and sham-operated mice. Picrosirius red was used to stain the left ventricle for collagen production. The cardiac output (CO), left ventricular end diastolic (LVEDD) and systolic (LVESD) diameter, and end-diastolic (LVEDV) and systolic (LVESV) volume was significantly increased at 7 and 21 days in AVF compared to sham-operated mice. There was also a significant increase in CO, LVEDD, LVESD, LVEDV, and LVESV from baseline to 21 days within the AVF group, but not the sham-operated mice. There was a significant decrease in ejection fraction and fractional shortening at 21 days in AVF compared to sham-operated mice. Picrosirius red was significantly more prominent around both the perivascular and interstitial areas of the cardiac tissue from AVF mice compared to sham-operated AVF mice at 21 days. The creation of an AVF in our murine model leads to cardiac changes such as increased cardiac output, left ventricular dilation, and cardiac fibrosis, while showing reductions of ejection fraction and fractional shortening.
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DOI：
10.1152/ajpheart.2001.280.2.h674
发表时间：
2001-02-01
影响因子：
4.8
作者：
Brower, GL;Janicki, JS
通讯作者：
Janicki, JS
影响因子：
24
作者：
Reddy, Yogesh N. V.;Melenovsky, Vojtech;Borlaug, Barry A.
通讯作者：
Borlaug, Barry A.
影响因子：
6.1
作者：
Korsheed, Shvan;Eldehni, Mohamed T.;McIntyre, Christopher W.
通讯作者：
McIntyre, Christopher W.
影响因子：
13.2
作者：
Movilli, Ezio;Viola, Battista Fabio;Cancarini, Giovanni
通讯作者：
Cancarini, Giovanni
影响因子：
13.2
作者：
Iwashima, Y;Horio, T;Kawano, Y
通讯作者：
Kawano, Y