Isolation of herbicidal substance, α-terthienyl, from the root of Flaveria bidentis using HSCCC and HPLC.
Isolation of herbicidal substance, α-terthienyl, from the root of Flaveria bidentis using HSCCC and HPLC.
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DOI：
10.17957/ijab/15.0080
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发表时间：
2015-10
影响因子：
--
通讯作者：
Hu Jingqian;Zhao Bin;Z. Zhe;Dong Jin-gao;Zhang Jinlin
中科院分区：
文献类型：
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作者：
Hu Jingqian;Zhao Bin;Z. Zhe;Dong Jin-gao;Zhang Jinlin
黄顶菊Flaveria bidentis（L.）昆茨是河北省一种具有化感作用的严重入侵植物。目的：从黄蜀葵根提取物中提取除草活性物质。采用高速逆流色谱（HSCCC）对黄顶菊的分离纯化方法进行了优化。结果表明：正己烷-乙酸乙酯-甲醇-水（6：4：5：5，v/v/v/v）的两相溶剂体系能将甘草根提取物分离，得到3个组分。除草活性筛选结果表明，组分3对马唐和拟南芥的除草活性最强。化合物3经高效液相色谱（HPLC）、核磁共振（NMR）和质谱（MS）鉴定为α-三噻吩基化合物。2016年12月28日-Friends Science Publishers
Flaveria bidentis (L.) Kuntze is a serious invasive plant with allelopathic properties in Hebei, China. To obtain the herbicidal substance from the root extracts of F. bidentis effectively and rapidly, the isolation and purification method was optimized by using high-speed counter-current chromatography (HSCCC). The results showed that the two-phase solvent system of nhexane-ethyl acetate-methanol-water (6:4:5:5, v/v/v/v) could separate the root extracts and get three components. The results of herbicidal activity screening for the three components indicated that component 3 had the strongest herbicidal activity against Digitaria sanguinalis and Arabidopsis thaliana. Component 3 was further separated and purified using highperformance liquid chromatography (HPLC) for structural determination, nuclear magnetic resonance (NMR) and mass spectrometry (MS) information validated the herbicidal componient 3 as α-terthienyl. © 2016 Friends Science Publishers