Microplate-based fluorometric methods for the enzymatic determination of L-glutamate: application in measuring L-glutamate in food samples
Microplate-based fluorometric methods for the enzymatic determination of L-glutamate: application in measuring L-glutamate in food samples
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DOI：
10.1016/s0003-2670(99)00533-4
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发表时间：
1999-12-03
影响因子：
6.2
通讯作者：
Zhou, MJ
中科院分区：
文献类型：
--
作者：
Chapman, J;Zhou, MJ
本报告描述了两种基于微孔板的荧光测定方法，用于在96孔微孔板测定中测量L-谷氨酸。这两种方法联合收割机结合了有利的酶循环和Amplex Red和刃天青的高灵敏度荧光指示剂。与现有的检测方法相比，基于Amplex Red的检测方法灵敏度高出500倍以上，检测限为0.5 pmol/孔，基于刃天青的检测方法灵敏度高出100倍以上，检测限为2.0 pmol/孔。这两种方法都被发现可以可靠地检测食品中的味精含量。由于其高灵敏度和所用指示剂的上级光谱特性，基于Amplex Red和刃天青的方法应可用于测定食品以及其他生物和临床样品中的L-谷氨酸水平。（C）1999 Elsevier Science B. V.保留所有权利。
This report describes two microplate-based fluorometric methods for measuring L-glutamate in a 96-well microplate assay. These two methods combine the advantageous enzymatic recycling and the highly sensitive fluorogenic indicators of Amplex Red and resazurin. In comparison with the existing assay, the Amplex Red-based assay is over 500-fold more sensitive with the detection limit of 0.5 pmol per well, and the resazurin-based assay is over 100-fold more sensitive with the detection limit 2.0 pmol per well. Both methods have been found to reliably detect MSG levels in foodstuffs. With their high sensitivity and the superior spectral properties of the indicators used, the Amplex Red- and resazurin-based methods should be useful for the determination of L-glutamate levels in foodstuffs, as well as in other biological and clinical samples. (C)1999 Elsevier Science B.V. All rights reserved.