Structure and function analysis in circulating tumor cells: using nanotechnology to study nuclear size in prostate cancer.
Structure and function analysis in circulating tumor cells: using nanotechnology to study nuclear size in prostate cancer.
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发表时间：
2018-04
影响因子：
1.2
通讯作者：
Nu Yao;Y. Jan;Shirley Cheng;Jie‐Fu Chen;L. Chung;H. Tseng;E. Posadas
中科院分区：
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作者：
Nu Yao;Y. Jan;Shirley Cheng;Jie‐Fu Chen;L. Chung;H. Tseng;E. Posadas
Donald Coffey教授和他的实验室开创了显示细胞核形状和细胞功能之间关系的研究。在这样做的过程中，他和他的学生建立了前列腺癌的核形态计量学领域。通过活检和手术取样使用围手术期组织，科菲博士的团队发现核形状和其他病理特征与临床结果指标相关。癌细胞也存在于实体瘤块之外，因为它们可以从原发性和转移性病变脱落到循环系统中。这些循环肿瘤细胞（CTC）的库是异质的。虽然这些CTC中的一些被动地流入循环，但其他CTC是具有侵袭潜力的主动转移者。纳米技术的进步现在使得通过液体活检研究血液中CTC的核形状等形态学特征成为可能。与传统的组织采样相比，液体活检允许微创、重复和系统性疾病采样，这克服了由于肿瘤时空异质性引起的疾病误报问题。我们的团队开发了一种新的液体活检方法，NanoVelcro测定，使我们能够识别CTC区室中的形态异质性。通过应用经典的核形态测量方法，我们在前列腺癌患者中鉴定了非常小的核CTC（vsnCTC）。我们的初步研究表明，vsnCTC与不利的临床行为（包括内脏转移的倾向）密切相关。这些方法可能会继续对动态临床行为产生更多的见解，这为更全面和准确的癌症分析创造了机会。最终，这些进步将使医生能够采用更准确和个性化的治疗方法，帮助该领域实现真正的精准医疗目标。
Professor Donald Coffey and his laboratory pioneered studies showing the relationships between nuclear shape and cellular function. In doing so, he and his students established the field of nuclear morphometry in prostate cancer. By using perioperative tissues via biopsies and surgical sampling, Dr. Coffey's team discovered that nuclear shape and other pathologic features correlated with clinical outcome measures. Cancer cells also exist outside of solid tumor masses as they can be shed from both primary and metastatic lesions into the circulatory system. The pool of these circulating tumor cells (CTCs) is heterogeneous. While some of these CTCs are passively shed into the circulation, others are active metastasizers with invasive potential. Advances in nanotechnology now make it possible to study morphologic features such as nuclear shape of CTCs in the bloodstream via liquid biopsy. Compared to traditional tissue sampling, liquid biopsy allows for minimally invasive, repetitive, and systemic disease sampling, which overcomes disease misrepresentation issues due to tumor temporospatial heterogeneity. Our team developed a novel liquid biopsy approach, the NanoVelcro assay, which allows us to identify morphologic heterogeneity in the CTC compartment. By applying classical methods of nuclear morphometry, we identified very small nuclear CTCs (vsnCTCs) in prostate cancer patients. Our initial studies showed that vsnCTCs strongly correlated with unfavorable clinical behaviors including the disposition to visceral metastases. These approaches may continue to yield additional insights into dynamic clinical behaviors, which creates an opportunity for more comprehensive and accurate cancer profiling. Ultimately, these advancements will allow physicians to employ more accurate and personalized treatments, helping the field reach the goal of true precision medicine.