Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites
Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites
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DOI：
10.1016/j.biomaterials.2003.08.029
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发表时间：
2004-05-01
期刊：
影响因子：
14
通讯作者：
Santerre, JP
中科院分区：
文献类型：
--
作者：
Finer, Y;Jaffer, F;Santerre, JP
研究表明，人唾液中含有胆固醇酯酶（CE）和拟胆碱酯酶（PCE）样水解酶活性。虽然PCE已被证明优先降解三甘醇二甲基丙烯酸酯（TEGDMA）及其衍生物，CE对复合牙科树脂中双酚A-二缩水甘油基二甲基丙烯酸酯（bisGMA）组分的分解具有更大的催化作用。目前的研究旨在确定是否有不同的酯酶之间的相互影响，就树脂复合材料的生物降解。将基于bisGMA/TEGDMA（his）或经硅烷改性的bisGMA/TEGDMA/bisEMA（ubis）单体的光聚合的模型复合树脂样品（含有60重量分数的硅烷化钡玻璃填料）在缓冲液、CE和/或PCE溶液（pH = 7.0，37 ℃）中孵育8天和16天。使用高效液相色谱法、紫外光谱法和质谱法分析孵育溶液中的降解产物。在his系统中，与两个单独的酶组的总和相比，在组合的酶组中检测到更高量（p < 0.05）的bisGMA衍生产物双羟基-丙氧基苯基-丙烷（bisHPPP）。在ubis系统中，类似的比较显示，在第8天时在组合组中检测到较高水平（p <0.05）的bisHPPP，而在第16天时在组合组中检测到较高量（p < 0.05）的bisEMA衍生产物乙氧基化双酚A。该研究得出结论，CE和PCE协同作用，以增加两种复合树脂材料的生物降解。(C)2003爱思唯尔有限公司。保留所有权利。
It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdown of bis-phenol-A-diglycidyl dimethacrylate (bisGMA) components in composite dental resins. The current study seeks to determine if there is a mutual influence between the different esterases with respect to the biodegradation of resin composite. Photopolymerized model composite resin samples (containing 60% by weight fraction of silanated barium glass filler) based on bisGMA/TEGDMA (his) or urethane-modified bisGMA/TEGDMA/bisEMA (ubis) monomers were incubated in buffer, CE and/or PCE solutions (pH = 7.0, 37degreesC for 8 and 16 days. The incubation solutions were analyzed for degradation products using high-performance liquid chromatography, UV spectroscopy and mass spectrometry. In the his system, higher amounts (p < 0.05) of a bisGMA derived product, bishydroxy-propoxyphenyl-propane (bisHPPP), were detected in the combined enzyme group as compared to the sum of the two individual enzyme groups. In the ubis system, similar comparisons showed that higher levels (p < 0.05) of bisHPPP were detected in the combined group at 8 days while higher amounts (p < 0.05) of a bisEMA derived product, ethoxylated bis-phenol A, were detected in the combined group at 16 days. The study concluded that CE and PCE act synergistically to increase the biodegradation of both composite resin materials. (C) 2003 Elsevier Ltd. All rights reserved.