The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes
The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes
复制标题
拟南芥转录因子 AtTCP15 通过调节关键细胞周期基因的表达来调节核内复制
DOI：
10.1093/mp/ssr086
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发表时间：
2012-01-01
期刊：
影响因子：
27.5
通讯作者：
Dong, Ai-Wu
中科院分区：
文献类型：
--
作者：
Li, Zi-Yu;Li, Bin;Dong, Ai-Wu
植物细胞经常经历核内复制，这是一种修饰的细胞周期，其中基因组重复复制而没有胞质分裂。核内复制是植物发育过程中细胞获得最终大小和功能的关键步骤。然而，控制核内复制和有丝分裂细胞分裂之间平衡的机制仍然知之甚少。在这里，我们表明，拟南芥TCP（类肉桂TEOSINTE BRANCHED 1-CYCLOIDEA-PCF）家族转录因子基因AtTCP 15在毛状体中表达，以及在快速分裂和维管组织。AtTCP 15 SRDX的表达，AtTCP 15与SRDX阻遏结构域融合，诱导转基因拟南芥中毛状体和子叶细胞的额外核内复制。相反，AtTCP 15的过表达抑制毛状体和其他检查细胞的核内复制。AtTCP 15的失调影响参与细胞周期调控的几个重要基因的表达。AtTCP 15蛋白直接与CYCA 2;3和视网膜母细胞瘤相关（RBR）基因的启动子区结合，这些基因在核内复制中起关键作用。综上所述，AtTCP 15在调节拟南芥发育过程中的核内复制中起着重要作用。
Plant cells frequently undergo endoreduplication, a modified cell cycle in which genome is repeatedly replicated without cytokinesis. As the key step to achieve final size and function for cells, endoreduplication is prevalent during plant development. However, mechanisms to control the balance between endoreduplication and mitotic cell division are still poorly understood. Here, we show that the Arabidopsis TCP (CINCINNATA-like TEOSINTE BRANCHED1-CYCLOIDEA-PCF)-family transcription factor gene AtTCP15 is expressed in trichomes, as well as in rapidly dividing and vascular tissues. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoreduplication in trichomes and cotyledon cells in transgenic Arabidopsis. On the contrary, overexpression of AtTCP15 suppresses endoreduplication in trichomes and other examined cells. Misregulation of AtTCP15 affects the expression of several important genes involved in cell-cycle regulation. AtTCP15 protein binds directly to the promoter regions of CYCA2;3 and RETINOBLASTOMA-RELATED (RBR) genes, which play key roles in endoreduplication. Taken together, AtTCP15 plays an important role in regulating endoreduplication during Arabidopsis development.