Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen
Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen
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介导成纤维细胞与胶原蛋白粘附的质膜糖蛋白
DOI：
10.1038/262497a0
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发表时间：
1976
期刊：
影响因子：
64.8
通讯作者：
E. Pearlstein
中科院分区：
文献类型：
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作者：
E. Pearlstein
与恶性肿瘤相关的细胞行为缺陷可能与转化时细胞质膜的改变有因果关系。许多兴趣集中在正常但未转化的细胞表面上发现的蛋白质2-7。该蛋白质命名为SF（参考文献5）、LETS（参考文献8）、带1（参考文献9）、L1（参考文献3）或CSP（参考文献10），是一种分子量为210，000 - 250，000的糖蛋白7、9、11（参考文献7、8和12）。它存在于血清中12，并且对通过低水平胰蛋白酶从正常成纤维细胞表面去除敏感2，3，9。已经描述了这种蛋白质的几种生物学特性。在有丝分裂中停滞的正常成纤维细胞缺乏这种蛋白质8，9。随着正常细胞接近融合，存在于细胞表面上的这种蛋白质的量的定量增加8，9，而融合的正常细胞，通过添加血清刺激，具有降低的水平8。与这些结果相矛盾的是，凝血酶对生长停滞的正常细胞的刺激导致胸苷掺入，而这种蛋白质的水平没有随之降低13。我们现在提出的证据指出，作为成纤维细胞的粘附胶原蛋白的介质的蛋白质的功能作用。该蛋白可能与先前报道的SV 40转化的3 T3成纤维细胞附着于胶原包被的培养皿所必需的血清因子相同14。基于这种功能作用，我们建议将这种蛋白质命名为细胞粘附因子（CAF）。
DEFECTS in cell behaviour associated with malignancy may be causally related to alterations in the plasma membrane of the cell on transformation1. Much interest has focused on a protein found on the surface of normal but not transformed cells 2–7. This protein, designated SF (ref. 5), LETS (ref. 8), band 1 (ref. 9), L1 (ref. 3) or CSP (ref. 10), is a glycoprotein7,9,11 with a molecular weight of 210,000–250,000 (refs 7, 8 and 12). It is present in the serum12 and is sensitive to removal from the surface of normal fibroblasts by low levels of trypsin2,3,9. Several biological properties of this protein have been described. Normal fibroblasts arrested in mitosis lack this protein8,9. As normal cells approach confluency, there is a quantitative increase in the amount of this protein present on the cell surface8,9, whereas confluent normal cells, stimulated by the addition of serum, have lowered levels8. In conflict with these results, implying a growth regulatory function, is the finding that stimulation of growth-arrested normal cells by thrombin leads to thymidine incorporation without a concomitant decrease in the levels of this protein13. We now present evidence which points to a functional role for the protein as a mediator of the adhesion of fibroblasts to collagen. The protein is probably identical to a serum factor previously reported to be necessary for the attachment of SV40-transformed 3T3 fibroblasts to collagen-coated dishes14. Based on this functional role, we propose the name cell adhesion factor (CAF) for this protein.