Agarose-acrylamide gradient gel electrophoresis of proteins.

Agarose-acrylamide gradient gel electrophoresis of proteins.
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蛋白质的琼脂糖-丙烯酰胺梯度凝胶电泳。

DOI:
10.1016/0003-2697(82)90489-4
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发表时间:
1982
影响因子:
2.9
通讯作者:
Fink,LM
Fink,LM
中科院分区:
生物学4区
文献类型:
--
作者:
Warren,DF;Naughton,MA;Fink,LM

文献摘要

相似文献

本文报道了一种新的琼脂糖-丙烯酰胺梯度平板凝胶电泳体系。这种新的凝胶的制备已通过使用具有相对低的凝胶化温度的琼脂糖而得到促进。分馏的标记蛋白和交联蛋白从亚细胞细胞骨架制备琼脂糖-丙烯酰胺梯度凝胶相比,发现使用其他丙烯酰胺凝胶电泳系统。
A new agarose-acrylamide gradient slab gel electrophoresis system is described. The preparation of this new gel has been facilitated by the use of agarose with a relatively low gelation temperature. Fractionation of marker proteins and crosslinked proteins from a subcellular cytoskeletal preparation on agarose-acrylamide gradient gels is compared to that found using other acrylamide gel electrophoresis systems.