Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver
Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver
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DOI：
10.1053/jhep.2001.21998
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发表时间：
2001-02-01
期刊：
影响因子：
13.5
通讯作者：
Stolz, DB
中科院分区：
文献类型：
--
作者：
Wack, KE;Ross, MA;Stolz, DB
比较了部分肝切除术（PHx）后大鼠肝再生过程中门静脉周围（1区）和中央周围（3区）区域的窦状隙内皮细胞（SEC）孔隙率。在对照肝脏以及PHx后5分钟、24、48、72、96、120小时和14天测量SEC孔隙率和开窗直径。在PHx后5分钟和5天，在两个区域中观察到孔隙率和开窗直径的双峰最大值。SEC孔隙率在PHx后5分钟内在1区和3区均显著增加，但仅在切除后24小时在1区保持增加。在初始上升之后，两个区域在PHx后72小时显示出逐渐降低至小于其孔隙度值的一半。72小时后，孔隙率增加至超过对照水平，并保持升高，直至PHx后14天。PHx后72小时的孔隙度降低伴随着此时窦状隙内的超微结构变化。血管腐蚀铸型和透射电子显微镜（TEM）显示，由于在SEC增殖不存在的情况下肝细胞增殖导致肝板宽度增加，导致血窦压缩。同样在这个时候，我们观察到许多SEC完全被星状细胞包裹。在整个再生过程中观察到的孔隙率的区域变化与层粘连蛋白，胶原蛋白I和IV，或纤维连接蛋白沉积的空间内的Disse的变化不相关。两者合计，数据显示，SEC是动态调节器的孔隙率，迅速和局部的环境带状刺激在肝再生。
Sinusoidal endothelial cell (SEC) porosities were compared between the periportal (zone 1) and pericentral (zone 3) regions of the rat liver during regeneration following partial hepatectomy (PHx). SEC porosities and fenestration diameters were measured in control livers, as well as at 5 minutes, 24, 48, 72, 96, 120 hours, and 14 days following PHx. Bimodal maximums in both porosity and fenestration diameters were observed in both zones at 5 minutes and 5 days following PHx. SEC porosities increased significantly in both zones 1 and 3 within 5 minutes following PHx, but the increase was maintained only in zone 1 at 24 hours after resection. Following the initial rise, both zones displayed a gradual decrease to less than half their porosity values at 72 hr post-PHx. After 72 hours, porosities increased to over control levels and remained elevated until 14 days after PHx. The decrease in porosity at 72 hr post-PHx is accompanied by ultrastructural changes within the sinusoid at this time. Vascular corrosion casting and transmission electron microscopy (TEM) show sinusoid compression resulting from increased hepatic plate widths due to hepatocyte proliferation in the absence of SEC proliferation. Also at this time, we observed many SEC completely enveloped by stellate cells. The zonal variations observed for porosities throughout regeneration did not correlate with changes in laminin, collagen I and IV, or fibronectin deposition within the space of Disse. Taken together, the data reveal that SEC are dynamic regulators of porosity that respond rapidly and locally to environmental zonal stimuli during liver regeneration.