Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport
Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport
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DOI：
10.1016/s0021-9290(03)00118-0
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发表时间：
2003-09-01
影响因子：
2.4
通讯作者：
Jacobs, CR
中科院分区：
文献类型：
--
作者：
Donahue, TLH;Haut, TR;Jacobs, CR
流体流动已被证明是调节骨细胞代谢的有效物理刺激。除了膜剪切应力之外，负载引起的流体流动还将增强由于对流或质量传输而导致的化学传输，从而影响细胞周围的生化环境。本研究研究了振荡流体流诱导的剪切应力和化学转运在骨细胞力转导机制中的作用。研究了不同剪切应力和化学转运水平下细胞内钙动员和前列腺素 E-2 (PGE(2)) 的产生。在这项研究中，MC3T3-E1 细胞对振荡流体流做出反应，细胞内钙浓度 ([Ca2+](i)) 增加，PGE(2) 产量增加。这些流体流动诱导的反应是通过化学转运来调节的。随着流速的增加，响应 [Ca2+](i) 振荡的细胞百分比增加，PGE(2) 的产生也是如此。此外，在液体流动过程中剥夺细胞的营养会导致 [Ca2+](i) 动员和 PGE(2) 产生受到抑制。这些数据表明，即使在恒定的峰值剪切应力下，剥夺细胞培养基中尚未确定的生化因子也会影响骨细胞的反应性。单独的化学转运不会引起反应，但似乎需要足够的营养供应或废物清除来响应振荡流体流引起的剪切应力。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Fluid flow has been shown to be a potent physical stimulus in the regulation of bone cell metabolism. In addition to membrane shear stress, loading-induced fluid flow will enhance chemotransport due to convection or mass transport thereby affecting the biochemical environment surrounding the cell. This study investigated the role of oscillating fluid flow induced shear stress and chemotransport in cellular mechanotransduction mechanisms in bone. Intracellular calcium mobilization and prostaglandin E-2 (PGE(2)) production were studied with varying levels of shear stress and chemotransport. In this study MC3T3-E1 cells responded to oscillating fluid flow with both an increase in intracellular calcium concentration ([Ca2+](i)) and an increase in PGE(2) production. These fluid flow induced responses were modulated by chemotransport. The percentage of cells responding with an [Ca2+](i) oscillation increased with increasing flow rate, as did the production of PGE(2). In addition, depriving the cells of nutrients during fluid flow resulted in an inhibition of both [Ca2+](i) mobilization and PGE(2) production. These data suggest that depriving the cells of a yet to be determined biochemical factor in media affects the responsiveness of bone cells even at a constant peak shear stress. Chemotransport alone will not elicit a response, but it appears that sufficient nutrient supply or waste removal is needed for the response to oscillating fluid flow induced shear stress. (C) 2003 Elsevier Science Ltd. All rights reserved.