The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome.
The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome.
复制标题
氧化/还原型谷胱甘肽的比率作为休克综合征各种实验模型中氧化应激的指标。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
D. Boda
中科院分区：
文献类型：
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作者：
I. Németh;D. Boda
红细胞能够很好地处理细胞内氧化应激，它们的膜可以渗透O2-和H2O2，因此它们是周围环境中发生的氧反应的重要调节剂。对各种组织内皮细胞损伤过程中产生的氧还原的保护作用主要归因于红细胞的谷胱甘肽代谢。采用谷胱甘肽还原酶敏感法测定百草枯毒性、止血带结扎和内毒素所致实验性休克综合征大鼠、狗出血再灌注和新生仔猪短暂结扎胸主动脉的血中还原性谷胱甘肽（GSH）和氧化性谷胱甘肽（GSSG）浓度。在这些条件下，GSSG浓度升高，GSH降低，导致氧化还原比增加：[GSSG/(GSH + GSSG)] × 100 (GSSG/GSH)；尤其是在再灌注过程中。测定GSSG/GSH似乎是评估“体内”氧化应激程度的可靠指标。
Red blood cells are well equipped to handle intracellular oxidative stress, their membranes are permeable to O2- and H2O2, and in this way they are important regulators of oxygen reactions occurring in their surroundings. The protective effect against reduced oxygen species - generated during the endothelial cell injury of various tissues - is attributed mainly to the glutathione metabolism of red blood cells. The blood concentration of reduced (GSH) and oxidized glutathione (GSSG) was determined by a sensitive method using glutathione reductase in experimental shock syndromes induced by paraquat toxicity, tourniquet ligature and endotoxin in rats, by bleeding and reperfusion in dogs and by transient ligature of thoracic aorta in newborn piglets. Under these conditions the concentration of GSSG was elevated and GSH was lowered, resulting in an increase in the redox ratio: [GSSG/(GSH + GSSG)] x 100 (GSSG/GSH); particularly during reperfusion. Determination of the GSSG/GSH seems to be a reliable index to assess the degree of oxidative stress "in vivo".