STRUCTURAL AND ANTIGENIC POLYMORPHISM OF THE 35-KILODALTON TO 48-KILODALTON MEROZOITE SURFACE-ANTIGEN (MSA-2) OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM
STRUCTURAL AND ANTIGENIC POLYMORPHISM OF THE 35-KILODALTON TO 48-KILODALTON MEROZOITE SURFACE-ANTIGEN (MSA-2) OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM
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DOI：
10.1128/mcb.11.2.963
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发表时间：
1991-02-01
影响因子：
5.3
通讯作者：
MCBRIDE, JS
中科院分区：
文献类型：
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作者：
FENTON, B;CLARK, JT;MCBRIDE, JS
人类寄生虫恶性疟原虫的裂殖子表面抗原 MSA-2 正在考虑用于开发疟疾疫苗。该抗原具有多态性，特异性单克隆抗体可区分 25 种寄生虫分离株中的 5 种 MSA-2 血清学变体。这些变体分为两个主要血清群，A 和 B。编码 A 血清群中两种不同变体的基因已被测序，并将它们的 DNA 和推导的氨基酸序列与其他等位基因编码的序列进行了比较。比较表明，血清学分类反映了 MSA-2 变体和血清群的 DNA 序列和推断的一级结构的差异。因此，同一血清群的变异体之间DNA和氨基酸序列的总体同源性超过95%。相比之下，A 组变体和 B 组变体之间的 DNA 和氨基酸序列相似性分别仅为 70% 和 64%。我们认为 MSA-2 蛋白是由两个高度不同的等位基因组编码的，每组中显示出有限的额外多态性。
Merozoite surface antigen MSA-2 of the human parasite Plasmodium falciparum is being considered for the development of a malaria vaccine. The antigen is polymorphic, and specific monoclonal antibodies differentiate five serological variants of MSA-2 among 25 parasite isolates. The variants are grouped into two major serogroups, A and B. Genes encoding two different variants from serogroup A have been sequenced, and their DNA together with deduced amino acid sequences were compared with sequences encoded by other alleles. The comparison shows that the serological classification reflects differences in DNA sequences and deduced primary structure of MSA-2 variants and serogroups. Thus, the overall homologies of DNA and amino acid sequences are over 95% among variants in the same serogroup. In contrast, similarities between the group A variants and a group B variant are only 70 and 64% for DNA and amino acid sequences, respectively. We propose that the MSA-2 protein is encoded by two highly divergent groups of alleles, with limited additional polymorphism displayed within each group.