DIFFUSIBLE AND BOUND ACTIN IN NUCLEI OF XENOPUS-LAEVIS OOCYTES
DIFFUSIBLE AND BOUND ACTIN IN NUCLEI OF XENOPUS-LAEVIS OOCYTES
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DOI：
10.1016/0092-8674(77)90152-0
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
MERRIAM, RW
中科院分区：
文献类型：
--
作者：
CLARK, TG;MERRIAM, RW
在人工分离的莱氏血吸虫卵母细胞核中，可以用几个标准来鉴定肌动蛋白，包括与肌动蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶上的共迁移、与抗肌动蛋白抗血清的免疫交叉反应、与DNA酶I的结合以及在十二烷基硫酸钠凝胶上的肽图谱。使用手工分离的细胞核排除了细胞质肌动蛋白污染或在分离过程中大量肌动蛋白从细胞核泄漏的可能性。肌动蛋白约占核蛋白总量的6%。在使用的核隔离条件下，大约75%的肌动蛋白是可扩散的。约25%稳定地与不溶性核凝胶结合，其中嵌入了染色体、核仁和其他核颗粒。肌动蛋白是核凝胶中最显著的单一成分，约占复合体总蛋白的16%。讨论了这些核内可扩散和结合的肌动蛋白的可能意义。
Several criteria may be used to identify actin in hand-isolated nuclei of X. laevis oocytes; these include co-migration with actin on SDS[sodium dodecyl sulfate]-polyacrylamide gels, immunological crossreactivity with antiserum against actin, binding to DNAase I and peptide mapping on SDS gels. The use of hand-isolated nuclei precludes the possibility of contamination from cytoplasmic actin or the leakage of significant amounts of actin from nuclei during isolation. Actin constitutes roughly 6% of the total nuclear protein. Approximately 75% of the actin is diffusible under the conditions of nuclear isolation used. About 25% is stably associated with an insoluble nuclear gel, in which chromosomes, nucleoli and other nuclear granules are embedded. Actin is the single most prominent component of the nuclear gel, comprising roughly 16% of the total protein of the complex. The possible significance of diffusible and bound actin in these nuclei is discussed.